FastQCFastQC Report
Sat 11 Mar 2023
EGAF00002637841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002637841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82417256
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG2749000.33354665435597614TruSeq Adapter, Index 13 (97% over 37bp)
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG1276640.15489960015169638No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG1265970.15360496835759735No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA1063120.12899240421204997No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA825920.10021202355972637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)