FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002637899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002637899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74397399
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG1044380.14037856350327518TruSeq Adapter, Index 2 (97% over 36bp)
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG1023940.13763115562682507No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA1022640.13745641833527003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)