FastQCFastQC Report
Thu 16 Mar 2023
EGAF00002637913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002637913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59865253
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG2063830.3447458912434564TruSeq Adapter, Index 7 (97% over 34bp)
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA822150.13733342110823452No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG768300.12833821983513544No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA664630.11102099576861389No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)