FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002638125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002638125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56892727
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG1396440.2454514089296511TruSeq Adapter, Index 13 (97% over 37bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG1235210.21711210995387864No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG995610.1749977637739179No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA831350.14612588354219688No Hit
AGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGTGTATGC693770.12194353067308586No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)