FastQCFastQC Report
Fri 2 Jun 2023
EGAF00002638217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002638217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63129186
Sequences flagged as poor quality0
Sequence length150-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG1046100.16570782331962905TruSeq Adapter, Index 6 (97% over 35bp)
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA725280.11488822301621313No Hit
GGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACA696850.11038475927758674No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)