FastQCFastQC Report
Fri 10 May 2024
EGAF00002641463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002641463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21930661
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG442280.201671987907706No Hit
GTAATATACAGCCGAGTCCTCAGCTCTCAGGCTGTTCATGTGCAGATACA439580.20044083486585287No Hit
GTATATTACTGTGCGAGTGGCGCTTCTGTTAAAATTGTGTATCCGGGGGA411630.18769612096963242No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT390040.17785145646088826No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC332360.15155037962604045No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG240290.10956806089884841No Hit
ATATGATACCTCACCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGCTCA235730.1074887802059409No Hit
CTATTACTGTCACCAATATGATACCTCACCTCTCACTTTCGGCGGAGGGA224650.1024364929082621No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG223310.10182547621341645No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG222440.10142877134437489No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)