FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002796521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002796521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467343
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCCCAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG10190.218041138949337No Hit
GTAGACCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG10150.21718523653932978No Hit
TTCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9460.20242091996670541No Hit
ATCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9330.19963923713418197No Hit
TTCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9240.1977134567116657No Hit
TGCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8790.18808455459908463No Hit
AGAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8690.1859447985740666No Hit
GTCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8570.18337709134404495No Hit
TACCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8340.1784556524865035No Hit
GGAGACCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8330.1782416768840017No Hit
AACCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8260.17674384766648907No Hit
ATCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8180.17503204284647464No Hit
AGCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8170.17481806724397284No Hit
TTAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8010.17139445760394398No Hit
TGCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8000.1711804820014422No Hit
GTCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7960.170324579591435No Hit
GTCCCCAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7800.16690096995140613No Hit
GACCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7660.1639053115163809No Hit
TACCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7600.1626214579013701No Hit
TGAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7500.16048170187635205No Hit
CGCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7450.15941182386384306No Hit
TTAGACCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7380.15791399464633044No Hit
CTCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7370.15770001904382863No Hit
GTCCCCAGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7210.15427640940379977No Hit
GGCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7200.15406243380129797No Hit
GGCCCCAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7190.15384845819879617No Hit
TTCCCCAGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6870.14700123891873848No Hit
GGAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6820.1459313609062295No Hit
CACCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6800.14550340970122588No Hit
GAAGACCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6740.14421955608621503No Hit
TTAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACCAGGAATCAGTCGA6720.14379160488121145No Hit
ATAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6630.14186582445869522No Hit
AGAGACCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6620.14165184885619342No Hit
GGCCCCAGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6610.14143787325369162No Hit
CGAGACCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6540.139940044036179No Hit
AGCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6530.1397260684336772No Hit
GGAGACCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6520.1395120928311754No Hit
ATCCCCAGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6480.13865619042116817No Hit
GACCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6470.1384422148186664No Hit
GTAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6420.13737233680615737No Hit
TGAGACCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6240.1335207759611249No Hit
ACCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6200.1326648735511177No Hit
TGAGACCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6130.13116704433360507No Hit
GAAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6130.13116704433360507No Hit
GAAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6090.13031114192359786No Hit
GAAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC6040.12924126391108884No Hit
TAAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6020.12881331270608526No Hit
ATAGACCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6000.12838536150108165No Hit
CGAGACCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5960.12752945909107444No Hit
GAAGACCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5940.12710150788607083No Hit
TAAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC5900.12624560547606362No Hit
TTAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC5870.12560367866855823No Hit
TTAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGGCAGCATGGGTAA5840.1249617518610528No Hit
TAAGACCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5790.12389187384854378No Hit
GACCCCAGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5750.12303597143853659No Hit
TCCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5740.12282199583603477No Hit
GGCCCCAGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5710.12218006902852936No Hit
TTAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5620.12025428860601313No Hit
AGAGACCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5600.11982633740100955No Hit
TGAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACCAGGAATCAGTCGA5560.11897043499100232No Hit
GTCCCCAGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5530.11832850818349692No Hit
TAAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACCAGGAATCAGTCGA5440.11640272776098069No Hit
GCCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5420.11597477655597707No Hit
AACCCCAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5410.11576080095347528No Hit
GTCCCCAGATGTTGCCAGGGTTTTCCCAGTCACGACGGCAGCATGGGTAA5390.11533284974847167No Hit
TAAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5360.11469092294096626No Hit
AACCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5350.11447694733846447No Hit
AAAGACCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5270.11276514251845003No Hit
GTCCCCAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACGGTTTTCTGT5250.11233719131344645No Hit
GGCCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGTGGTCGCTATTCT5240.11212321571094463No Hit
TTCCCCAGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5220.11169526450594103No Hit
TTAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5210.11148128890343922No Hit
TAAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGGCAGCATGGGTAA5170.11062538649343201No Hit
GGCCCCAGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5140.1099834596859266No Hit
GTAGACCGACTCAGCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC5140.1099834596859266No Hit
CGAGACCGATAAGCCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5130.10976948408342481No Hit
CGAGACCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5110.1093415328784212No Hit
ATCCCCAGATGTTGCCAGGGTTTTCCCAGTCACGACGGCAGCATGGGTAA5100.10912755727591941No Hit
CAAGACCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5090.1089135816734176No Hit
TACCCCAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5080.1086996060709158No Hit
CGAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5080.1086996060709158No Hit
CTAGACCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5060.10827165486591218No Hit
TTAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGGAATCAGGACTCA5060.10827165486591218No Hit
TAAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5030.10762972805840679No Hit
GACCCCAGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG4990.10677382564839956No Hit
TGCCCCAGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG4900.10484804522588335No Hit
GTCCCCAGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG4890.10463406962338154No Hit
CTCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG4880.10442009402087975No Hit
TTCCCCAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACGGTTTTCTGT4870.10420611841837793No Hit
GGCCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC4850.10377816721337434No Hit
AAAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG4850.10377816721337434No Hit
TTCCCCAGATGTTGCCAGGGTTTTCCCAGTCACGACGGCAGCATGGGTAA4830.10335021600837073No Hit
TCCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG4820.10313624040586891No Hit
TGAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG4810.10292226480336712No Hit
CTAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG4800.10270828920086533No Hit
GTCCCCAGATGTTGCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC4780.10228033799586171No Hit
AAAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG4780.10228033799586171No Hit
AGCCCCAGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG4780.10228033799586171No Hit
ATCCCCAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG4740.1014244355858545No Hit
TGAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGGCAGCATGGGTAA4720.10099648438085089No Hit
GTAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC4710.1007825087783491No Hit
CACCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG4690.10035455757334548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)