FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002796525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002796525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516945
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG14240.2754645078296531No Hit
CGTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG13580.2626971921577731No Hit
CTATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG13130.25399220419967306No Hit
ATATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG12870.24896265560165973No Hit
ATTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG12790.24741510218688642No Hit
TTTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG12500.2418052210583331No Hit
AGTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG11960.2313592355086131No Hit
TCATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG11350.21955914072096647No Hit
TTATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG10540.20389016239638647No Hit
GTATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG10290.1990540579752198No Hit
GTTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG10250.19828028126783315No Hit
TGTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG10130.19595895114567313No Hit
TAATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG10130.19595895114567313No Hit
TTTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9990.1932507326698198No Hit
TTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9580.1853195214191065No Hit
GGTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9250.1789358635831665No Hit
CAATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9240.17874241940631982No Hit
AAATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9030.1746800916925398No Hit
CTTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8380.1621062201975065No Hit
GTTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8330.16113899931327316No Hit
TGTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8200.1586242250142665No Hit
ACATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8190.15843078083741985No Hit
CTATGTCGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8110.15688322742264652No Hit
TTATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8100.15668978324579985No Hit
GTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8080.15630289489210653No Hit
CATCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7890.15262745553201984No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7860.15204712300147985No Hit
GTTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7860.15204712300147985No Hit
ATTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7690.14875857199508652No Hit
CCATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7630.1475979069340065No Hit
TTTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7630.1475979069340065No Hit
TATCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7620.14740446275715985No Hit
CCATGTCGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7550.14605035351923318No Hit
GTATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7500.14508313263499986No Hit
TGATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7460.14430935592761318No Hit
CTTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7440.14392246757391985No Hit
AATCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7350.14218146998229986No Hit
CTATCCTGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7210.13947325150644654No Hit
TTATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7180.13889291897590653No Hit
GCATCCTGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7170.13869947479905986No Hit
TGATCCTGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7150.13831258644536654No Hit
TTATCCTGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7120.13773225391482655No Hit
TTATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7120.13773225391482655No Hit
CGATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7110.13753880973797986No Hit
TTATGTCGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7070.1367650330305932No Hit
CTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7020.13579781214635986No Hit
GTATCCTGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6990.13521747961581987No Hit
TGATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6960.13463714708527988No Hit
TCATGTCGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6950.1344437029084332No Hit
TATCACGGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6920.1338633703778932No Hit
CGTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6880.13308959367050655No Hit
GGTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6870.13289614949365985No Hit
GATCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6850.13250926113996656No Hit
ATTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC6840.13231581696311986No Hit
TATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6780.13115515190203986No Hit
CGATCCTGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6720.12999448684095988No Hit
GTATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6680.1292207101335732No Hit
TTATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC6670.12902726595672653No Hit
TGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6640.12844693342618654No Hit
TCATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6600.12767315671879986No Hit
TTATCCTGACTCAGCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC6570.12709282418825987No Hit
CTATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6470.1251583824197932No Hit
CCTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6390.12361082900501988No Hit
GGATCCTGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6370.12322394065132655No Hit
TTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC6370.12322394065132655No Hit
TATCACGGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6340.12264360812078655No Hit
TCATCCTGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6310.12206327559024656No Hit
TTTCACGGATGTTGCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC6300.12186983141339988No Hit
ATATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6290.1216763872365532No Hit
TTTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC6240.12070916635231989No Hit
TGATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6240.12070916635231989No Hit
ATTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6110.11819439205331321No Hit
AGTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6100.11800094787646656No Hit
TTATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACGGAATCAGGACTCA6050.11703372699223322No Hit
CTATGTCGAGGAATCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC6040.11684028281538655No Hit
CTATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6010.11625995028484656No Hit
GCATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG6010.11625995028484656No Hit
AGATGTCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5950.11509928522376654No Hit
CTTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5890.11393862016268655No Hit
TTATGTCGACGAGGCCAGGGTTTTCCCAGTCACGACGGAATCAGGACTCA5840.11297139927845322No Hit
ATTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5840.11297139927845322No Hit
GGTCACGGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5780.11181073421737323No Hit
AATCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5720.11065006915629322No Hit
TCATGTCGAGGAATCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC5670.1096828482720599No Hit
AGTCACGGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5650.10929595991836655No Hit
TAATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5640.1091025157415199No Hit
GTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC5620.10871562738782654No Hit
CCATCCTGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5610.1085221832109799No Hit
GATCACGGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5590.10813529485728655No Hit
TGTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5590.10813529485728655No Hit
CAATGTCGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5510.10658774144251322No Hit
TTTCACGGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5470.10581396473512655No Hit
TTATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACGGAATCAGGACTCA5460.1056205205582799No Hit
TATCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5440.10523363220458655No Hit
TCTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5360.10368607878981323No Hit
CGTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC5350.10349263461296655No Hit
TTATGTCGAGGAATCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC5340.1032991904361199No Hit
AGTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5330.10310574625927323No Hit
GTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5330.10310574625927323No Hit
CATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5250.1015581928444999No Hit
TTATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC5240.10136474866765323No Hit
TTATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGGAATCAGGACTCA5220.1009778603139599No Hit
ACTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG5220.1009778603139599No Hit
GTTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACTCAGGAGTCAGTCC5180.10020408360657324No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)