FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002796543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002796543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719739
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG16490.2291108304538173No Hit
TTTCGAAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG14300.19868313374709445No Hit
TTGGTGCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG12410.1724236146714295No Hit
TTTCGAAGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG10850.15074909098992828No Hit
TATCGAAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG10430.1449136423064472No Hit
TCGGTGCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG10310.14324637125402404No Hit
TTGGTGCGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG10120.14060652542102067No Hit
TCGGTGCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG10090.14018970765791489No Hit
ATTCGAAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9840.13671622629869995No Hit
TTTCGAAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9560.1328259271763792No Hit
TTGCAGAGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9500.13199229165016763No Hit
GTTCGAAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9150.12712941774726672No Hit
TTTCGAAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9150.12712941774726672No Hit
ATGGTGCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG9080.12615684296668653No Hit
TTGCAGAGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8980.12476745042300057No Hit
TGTCGAAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8980.12476745042300057No Hit
TTAACAAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8950.12435063265989477No Hit
TCGGTGCGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8940.12421169340552617No Hit
TCGGTGCGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8940.12421169340552617No Hit
TTAACAAGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8930.12407275415115757No Hit
TTTCGAAGATGTTGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8800.1222665438443658No Hit
TTGGTGCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8790.1221276045899972No Hit
CCGGTGCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8740.12143290831815423No Hit
TTGCAGAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8730.12129396906378563No Hit
TATCGAAGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8670.12046033353757404No Hit
TTGCAGAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8580.11920988024825667No Hit
TTTCGAAGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8540.11865412323078227No Hit
ATAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8520.11837624472204507No Hit
TTTCGAAGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8450.11740366994146488No Hit
TTAACAAGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8410.11684791292399051No Hit
CCGGTGCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8320.11559745963467313No Hit
TTGGTGCGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8270.11490276336283015No Hit
TAGGTGCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG8020.11142928200361521No Hit
TCGGTGCGACAACTCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7900.10976201095119202No Hit
CTGGTGCGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7890.10962307169682342No Hit
TGTCGAAGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7870.10934519318808625No Hit
CTGGTGCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7820.10865049691624325No Hit
GTAACAAGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7680.10670534735508289No Hit
TTAACAAGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7660.10642746884634569No Hit
ACGGTGCGATGCACCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7620.10587171182887131No Hit
ATTCGAAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7530.10462125853955392No Hit
GTAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7530.10462125853955392No Hit
ATTCGAAGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7520.10448231928518532No Hit
GTAACAAGACGAGGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7510.10434338003081672No Hit
TTAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACGGTTTTCTGT7390.10267610897839356No Hit
CTAACAAGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7390.10267610897839356No Hit
GTTCGAAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7240.10059202016286459No Hit
TGAACAAGACTCAGCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7230.100453080908496No Hit
TTGCAGAGAGGAATCCAGGGTTTTCCCAGTCACGACAGGAATCAGCCCAG7200.1000362631453902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)