FastQCFastQC Report
Fri 13 Jan 2023
EGAF00002796646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002796646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369974868
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88921792.403454874670028Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14750780.39869681094122317Clontech SMART CDS Primer II A (100% over 24bp)
CTTCGATGTTGTGCTCTCCCAGTCTCACCTGGATGCGGGACTTGTAGCAG9243210.2498334562551962No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7358900.19890269952066042Clontech SMART CDS Primer II A (100% over 25bp)
GGGACTTGTAGCAGTGGCCTGCTGATACCACCCACTGTTCGTTGATGAGG6325130.17096107187475232No Hit
GTAGCAGTGGCCTGCTGATACCACCCACTGTTCGTTGATGAGGGAGCCAC5252010.1419558584719868No Hit
GGCCTGCTGATACCACCCACTGTTCGTTGATGAGGGAGCCACCACAGAAG4777430.12912850069589052No Hit
CCCAGTCTCACCTGGATGCGGGACTTGTAGCAGTGGCCTGCTGATACCAC4314270.1166098125346179No Hit
CCAGGACTTCGATGTTGTGCTCTCCCAGTCTCACCTGGATGCGGGACTTG4013260.10847385449976024No Hit
GTTGTGCTCTCCCAGTCTCACCTGGATGCGGGACTTGTAGCAGTGGCCTG4001100.1081451835263578No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3927240.10614883170929329Clontech SMART CDS Primer II A (100% over 23bp)
CTCCCAGTCTCACCTGGATGCGGGACTTGTAGCAGTGGCCTGCTGATACC3867010.10452088329415933No Hit
CTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCGATGTTGT3762390.10169312365292876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)