FastQCFastQC Report
Sat 25 May 2024
EGAF00002796652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002796652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78608594
Total Bases11.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13302321.6922220997872064Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2344970.29830962248224413Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1233800.15695484898254256Clontech SMART CDS Primer II A (100% over 25bp)
CTTCGATGTTGTGCTCTCCCAGTCTCACCTGGATGCGGGACTTGTAGCAG1052190.1338517770716011No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC822080.10457889629726744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)