FastQCFastQC Report
Mon 6 Feb 2023
EGAF00002796654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002796654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122432420
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19630121.6033432974697388Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3525140.28792537140081037Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1827110.14923416526439648Clontech SMART CDS Primer II A (100% over 25bp)
CTTCGATGTTGTGCTCTCCCAGTCTCACCTGGATGCGGGACTTGTAGCAG1650950.13484581943246732No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC1286830.10510533076124771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)