FastQCFastQC Report
Tue 24 Jan 2023
EGAF00002796656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002796656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143093497
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23788191.6624228562951395Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4241530.29641668481971617Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2202170.15389728018178214Clontech SMART CDS Primer II A (100% over 25bp)
CTTCGATGTTGTGCTCTCCCAGTCTCACCTGGATGCGGGACTTGTAGCAG1936470.1353290010097384No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC1503110.10504390706168848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)