FastQCFastQC Report
Thu 16 May 2024
EGAF00002796658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002796658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58629646
Total Bases8.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9777631.6676938489446107Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1741760.29707837567363105Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT906780.1546623699552953Clontech SMART CDS Primer II A (100% over 25bp)
CTTCGATGTTGTGCTCTCCCAGTCTCACCTGGATGCGGGACTTGTAGCAG790900.13489762500015776No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC613040.10456143637640249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)