FastQCFastQC Report
Mon 18 Sep 2023
EGAF00002796729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002796729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60073621
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2151990.3582254514007071No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1779580.2962331836131536TruSeq Adapter, Index 9 (100% over 50bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1569860.26132268604218145No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1032350.17184747361907818No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1028780.17125320279927858No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG817580.1360963408548321No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT809010.13466975796248407No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA716260.11923036901671034No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC691980.11518866159241509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)