FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002796731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002796731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63879203
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA4238730.6635539895511846No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2719020.4256502699321405No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1986490.31097601515159795No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1812420.28372614479864444No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1783000.2791205770053205No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1490570.2333419845579476No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1489010.233097773621252No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1446540.2264492874151858No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1398400.21891318838151438No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC1391660.21785807189861153TruSeq Adapter, Index 10 (100% over 50bp)
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1216110.1903765142467416No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1185210.18553925915450134No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1091890.17093043568499125No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1091240.17082868112803473No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC997040.15608209764295275No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG920880.14415959447709453No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT910390.14251743247328869No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT868230.13591747536361717No Hit
CTCGATGTGAATGATGCTTTTCATGGAATTTTCTTTTATACCCATGATGT858120.1343348006392628No Hit
CTCCATTCCACTTGCGAATAACTGTGCCGAGTTTTGCACCATACTTGAAT845420.13234667314180484No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC833300.13044934201824654No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT776180.12150746464385286No Hit
GTGGCTTTCCCTGAGGAGGTGGTGGACCTTGTTGCTGCTGGCCTCCTTGT714800.11189870355771345No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT701550.10982447605052306No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG690180.10804455403114531No Hit
GCAAGTATCTAATCAAATGATAATTAGGAAGGGAAGTATCCTGAAACACA679150.10631785747232944No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT674490.10558835557168739No Hit
GTTGGTATTGTGGTTGGTATGGTTGTGGGTATAGTGGTTGTTCTGGAACT666760.1043782590712661No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA649670.1017028969506711No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG645320.10102192414642368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)