FastQCFastQC Report
Tue 8 Aug 2023
EGAF00002796756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002796756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68758394
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT4811550.6997763793028674No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC3364240.48928426106054773No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT3205020.4661278156089568No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG3110440.4523724041605742No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTC2554730.37155172646993473No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC2111230.3070505108074514No Hit
GATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA2054770.29883914973348563No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT1981980.2882528059046871No Hit
CTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAAC1976090.287396183220917No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1961450.2852669886385072No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC1940850.2822709907971382No Hit
CGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTC1933120.2811467644226827No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1925290.28000799436938567No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA1911900.27806059577249576No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT1792700.26072453059331197No Hit
GGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCT1720550.25023126630909964No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1690160.24581144230913826No Hit
GTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGA1647280.23957511282186145No Hit
GAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC1618880.23544470803084785No Hit
ATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAAT1544310.22459948671866886No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG1481210.21542242536962106No Hit
CACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGG1452680.21127311379611338No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA1439010.20928499289846708No Hit
CTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGT1438180.20916428036408177No Hit
GTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCC1422860.20693618876554912No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATA1417320.20613046895772463No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG1386300.20161901978106123No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT1371360.1994461941621266No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC1360570.19787693121511826No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT1311730.1907737984688822No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC1294480.1882650138687067No Hit
GGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAA1290470.18768181234715867No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA1287750.18728622428266722No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATC1277070.18573295938238465No Hit
TGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGA1273860.18526610729156937No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT1235300.1796580647302495No Hit
ACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAAC1226260.1783433161629691No Hit
CATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATA1218470.1772103635812087No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT1211650.17621848468421178No Hit
CTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAA1207730.1756483724736212No Hit
CAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA1195550.17387695239071466No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC1192210.1733911935174053No Hit
GAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACA1172390.17050863637100075No Hit
CCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTA1160220.1687386706559784No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC1154230.16786750429336672No Hit
CCTAGCCTTTCTATTAGCTCTCAGTAAGATTACACATGCAAGCATCCCCG1144550.1664596761815001No Hit
CGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAG1123580.16340986672841717No Hit
AGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAA1104370.1606160260229464No Hit
CCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT1098480.1597594033391763No Hit
CCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGA1073500.15612639236454534No Hit
CCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATT1068350.15537739290420308No Hit
CAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAA1067430.15524359105886038No Hit
TTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAAC1027790.1494784767660513No Hit
AACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAA1011130.1470554998710412No Hit
AATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAA1006090.1463224984574247No Hit
GAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTA994120.14458162010008552No Hit
TCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAA992510.1443474668707358No Hit
AAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGC980450.1425934992024392No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG965310.1403915862258214No Hit
GGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT963010.14005708161246463No Hit
CAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTA919160.1336796784404243No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA916460.13328699911170117No Hit
CAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC903960.13146903925650152No Hit
GTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGT902960.13132360246808555No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC900960.1310327288912536No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT897400.13051497392449277No Hit
CAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA886630.12894861971325275No Hit
GAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTA885320.12875809752042783No Hit
AGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAA881660.12822579887482538No Hit
ATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTT877010.12754951780869112No Hit
CCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA875450.1273226364187622No Hit
GATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT870600.12661726799494474No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA848100.12334494025558539No Hit
ATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAAT833440.12121283693740724No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA832400.12106158267745463No Hit
CCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATA823550.11977446709997328No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC816100.1186909630262743No Hit
ATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAG811780.1180626761003173No Hit
ACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGA811590.11803504311051825No Hit
GCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAA806750.11733112905458495No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC800990.11649341315330897No Hit
CACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCA797720.11601783485518874No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC795520.11569787392067359No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC766720.11150929441429362No Hit
CCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACT766140.11142494107701235No Hit
AGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAG760290.11057413586477893No Hit
CCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACG752110.10938446293553628No Hit
TAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGA749180.10895833314547747No Hit
CGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTAT738640.10742542939557315No Hit
ATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAAC738520.10740797698096322No Hit
CGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCT738500.10740506824519491No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA726570.10567000735939237No Hit
AGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGAT723620.10524096883356526No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCA722440.1050693534232344No Hit
CTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGA717480.10434798695269118No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA709180.10314086160883862No Hit
AGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTC706510.10275254538376798No Hit
ATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAG693120.10080514678687813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)