FastQCFastQC Report
Sat 1 Apr 2023
EGAF00002797479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002797479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29054750
Sequences flagged as poor quality0
Sequence length65
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTATGCCGTCTTCTGCTTG12101934.1652156704153365TruSeq Adapter, Index 25 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTATGCCGTCTTCTGCTTGA760880.26187800617799156TruSeq Adapter, Index 25 (97% over 37bp)

[OK]Adapter Content

Adapter graph