FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002797509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002797509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40860731
Sequences flagged as poor quality0
Sequence length65
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTATGCCGTCTTCTGCTTG1304562431.927045064367547TruSeq Adapter, Index 6 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTATGCCGTCTTCTGCTTGA4706921.1519421911468006TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCACATAATCTCGTATGCCGTCTTCTGCTTG1106790.2708688691839605TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCATATAATCTCGTATGCCGTCTTCTGCTTG533210.13049448381136403TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACACAATCTCGTATGCCGTCTTCTGCTTG417450.10216410469993795TruSeq Adapter, Index 6 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)