FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002797927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002797927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32500
Sequences flagged as poor quality0
Sequence length32-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGAATATTGTACGGTACCATAAATACTTGACCACCTGTAGTACATAA530.16307692307692306No Hit
ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAG510.15692307692307692No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN480.14769230769230768No Hit
GTTAAGGGTGGGTAGGTTTGTTGGTATCCTAATGGGTGAGGGGTGACTTT460.14153846153846156No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT430.13230769230769232No Hit
TCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTAT430.13230769230769232No Hit
CTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC410.12615384615384614No Hit
CTTGAAACTAGGCGGCTATGGTATAATACGCCTCACACTCATTCTCAACC390.12No Hit
GTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAG380.11692307692307692No Hit
CTAGTATATCGCTCACACCTCATATCCTCCCTACTATGCCTAGAAGGAAT360.11076923076923079No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG340.10461538461538462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)