FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002798169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21935
Sequences flagged as poor quality0
Sequence length32-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGAATATTGCACGGTACCATAAATACTTGACCACCTGTAGTACATAA390.17779803966263963No Hit
ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAG380.1732391155687258No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT310.14132664691132893No Hit
TCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTAT300.1367677228174151No Hit
GTTGAAATCTCCTAAGTGTAAGTTGGGTGCTTTGTGTTAAGCTACACTCT250.11397310234784591No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT250.11397310234784591No Hit
CTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC250.11397310234784591No Hit
GCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT240.10941417825393207No Hit
GTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAG220.1002963300661044No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA220.1002963300661044No Hit
GCTGAAGATGGCGGTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGG220.1002963300661044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)