FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002798257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55074
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCC7141.2964375204270617No Hit
GAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTT6871.2474125721756184No Hit
AAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCT3810.6917964919925917No Hit
TTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAG2900.5265642589969859No Hit
CTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAG2840.5156698260522207No Hit
CCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTT2820.5120383484039656No Hit
GCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGC2730.49569669898681773No Hit
GAGCCAACCTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTT2580.4684606166249047No Hit
GCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGC2540.46119766132839457No Hit
GCCAACCTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGC2330.42306714602171624No Hit
TTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTG1620.29414968950866105No Hit
CTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAG1590.2887024730362785No Hit
ACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGA1430.2596506518502379No Hit
TGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGC1360.2469404800813451No Hit
AGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAA1300.2360460471365799No Hit
GGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAAC1180.21425718124704943No Hit
TCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGT1170.21244144242292187No Hit
GAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAACCTTT1110.20154700947815668No Hit
GAGCCAAACTTTGCAAGGAGAGCCAACCTTTGCAAGGAGAGCCAAACTTT1000.18157388241275377No Hit
GGTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCC960.1743109271162436No Hit
TTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGA950.17249518829211608No Hit
GTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGGTTGGCTCTCC910.1652322329956059No Hit
AGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTC890.16160075534735083No Hit
GAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACT810.14707484475433052No Hit
GTTTGGCTCTCCTTGCAAAGGTTGGCTCTCCTTGCAAAGTTTGGCTCTCC750.1361804118095653No Hit
AGCCAACCTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTG650.11802302356828995No Hit
CCTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGA590.10712859062352471No Hit
GCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCC580.10531285179939717No Hit
GAGCCAACCTTTGCAAGGAGAGCCAACCTTTGCAAGGAGAGCCAAACTTT570.10349711297526965No Hit
CAACCTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAA570.10349711297526965No Hit
AAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGGTTGGCTCT560.10168137415114209No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)