FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002798258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55074
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCC9731.7667138758760939No Hit
GAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTT6551.1893089298035369No Hit
AAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCT3510.6373243272687656No Hit
CCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTT3250.5901151178414497No Hit
GCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGC2570.4666448778007771No Hit
TTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAG2440.4430402730871192No Hit
CTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAG2400.43577731779060896No Hit
GCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGC2370.4303301013182264No Hit
GAGCCAACCTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTT2270.412172713076951No Hit
GCCAACCTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGC1550.28143951773976833No Hit
CTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAG1540.2796237789156408No Hit
TTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTG1390.2523876965537277No Hit
TCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGT1310.23786178596070742No Hit
ACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGA1310.23786178596070742No Hit
GGTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCC1300.2360460471365799No Hit
AGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAA1290.23423030831245234No Hit
GTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGGTTGGCTCTCC1290.23423030831245234No Hit
AGTTTGGCTCTCCTTGCAAAGTTTGGCTCTCCTTGCAAAGTTTGGCTCTC1250.22696735301594217No Hit
GTTTGGCTCTCCTTGCAAAGGTTGGCTCTCCTTGCAAAGTTTGGCTCTCC1230.22333587536768712No Hit
TGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGC1100.19973127065402912No Hit
GGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAAC1010.1833896212368813No Hit
GAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACT870.15796927769909577No Hit
GAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAACCTTT810.14707484475433052No Hit
TTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTGCAAGGAGA780.14162762828194791No Hit
GAGCCAAACTTTGCAAGGAGAGCCAACCTTTGCAAGGAGAGCCAAACTTT720.1307331953371827No Hit
AGCCAACCTTTGCAAGGAGAGCCAAACTTTGCAAGGAGAGCCAAACTTTG680.12347024004067254No Hit
GAGCCAACCTTTGCAAGGAGAGCCAACCTTTGCAAGGAGAGCCAAACTTT650.11802302356828995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)