FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002798355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28231
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA650.23024334950940456No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC530.1877368849845914No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA520.18419467960752364No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT470.16648365272218482No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG390.13814600970564272No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA390.13814600970564272No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT380.13460380432857497No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT360.12751939357443945No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT350.12397718819737169No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA350.12397718819737169No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG350.12397718819737169No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC350.12397718819737169No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT340.12043498282030393No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC330.11689277744323615No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC330.11689277744323615No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC320.11335057206616839No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC320.11335057206616839No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA300.10626616131203287No Hit
CCCTTAACAGTACATAGTACATAAAGCCATTTACCGTACATAGCACATTA300.10626616131203287No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN290.10272395593496511No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC290.10272395593496511No Hit
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT290.10272395593496511No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT290.10272395593496511No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG290.10272395593496511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)