FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002798356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28231
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA770.2727498140342177No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT470.16648365272218482No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA450.1593992419680493No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC440.15585703659098155No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT400.14168821508271048No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG370.1310615989515072No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA360.12751939357443945No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT360.12751939357443945No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG360.12751939357443945No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG340.12043498282030393No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC330.11689277744323615No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG310.10980836668910063No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG290.10272395593496511No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC290.10272395593496511No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN290.10272395593496511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)