FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002798359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25617
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN610.23812312136471872No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA420.1639536245461998No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT360.14053167818245696No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA350.1366280204551665No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT350.1366280204551665No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG320.12491704727329508No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC300.11710973181871415No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC300.11710973181871415No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT300.11710973181871415No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT290.11320607409142366No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC290.11320607409142366No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA280.1093024163641332No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA270.10539875863684273No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC260.10149510090955226No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC260.10149510090955226No Hit
CATATAACCTCCCCCAAAATTCAGAATAATAACACACCCGACCACACCGC260.10149510090955226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)