FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002798360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25617
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN600.2342194636374283No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA510.19908654409181403No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT370.14443533590974744No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA360.14053167818245696No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC360.14053167818245696No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT340.13272436272787602No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG330.12882070500058557No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT320.12491704727329508No Hit
CCCTTAACAGTACATAGTACATAAAGCCATTTACCGTACATAGCACATTA320.12491704727329508No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG300.11710973181871415No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC290.11320607409142366No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT280.1093024163641332No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA270.10539875863684273No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC270.10539875863684273No Hit
TCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGCCAA270.10539875863684273No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA270.10539875863684273No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA260.10149510090955226No Hit
CTAGTATATCGCTCACACCTCATATCCTCCCTACTATGCCTAGAAGGAAT260.10149510090955226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)