FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002798373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23711
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN440.1855678798869723No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA410.17291552444013328No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC360.15182826536206825No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT320.1349584580996162No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT310.1307410062840032No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC300.1265235544683902No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT300.1265235544683902No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG300.1265235544683902No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC290.12230610265277718No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA280.11808865083716417No Hit
CTTCTAGCAAGCCTCGCTAACCTCGCCTTACCCCCCACTATTAACCTACT280.11808865083716417No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC270.11387119902155117No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA270.11387119902155117No Hit
TCCTAGACCTAACCTGACTAGAAAAGCTATTACCTAAAACAATTTCACAG260.10965374720593817No Hit
CATATAACCTCCCCCAAAATTCAGAATAATAACACACCCGACCACACCGC260.10965374720593817No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA250.10543629539032516No Hit
CTAGTATATCGCTCACACCTCATATCCTCCCTACTATGCCTAGAAGGAAT250.10543629539032516No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT250.10543629539032516No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA250.10543629539032516No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG250.10543629539032516No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC240.10121884357471216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)