FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002798580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97199
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2420.24897375487402135No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT1990.204734616611282No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1960.20164816510457925No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG1930.19856171359787655No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1830.18827354190886736No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG1820.18724472473996645No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA1550.15946666117964176No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT1510.15535139250403812No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA1450.14917848949063262No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC1430.1471208551528308No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC1390.14300558647722714No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG1390.14300558647722714No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1370.1409479521394253No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT1280.13168859761931706No Hit
CATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTG1280.13168859761931706No Hit
TTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCA1240.12757332894371343No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1160.1193427915925061No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT1150.11831397442360518No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG1130.11625634008580335No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT1120.11522752291690244No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1120.11522752291690244No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC1110.11419870574800152No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT1100.11316988857910061No Hit
GTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGC1100.11316988857910061No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG1080.11111225424129878No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG1050.10802580273459603No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT1050.10802580273459603No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG1040.10699698556569512No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT1010.10391053405899237No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA1010.10391053405899237No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG990.10185289972119053No Hit
GCATTAGGAATGCCATTGCGATTAGAATGGGTACAATGAGGAGTAGGAGG980.10082408255228963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)