FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15562557
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAATGCACAGGCTGTGAACTCGAAGAGTAGGGCGGGACACGTGGTAT584280.3754395887513858No Hit
TGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTT524180.3368212563012621No Hit
GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA488960.31419001389039086No Hit
AGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGGAAACGATGTGGGAAGGCACAGAC384690.2471894560771729No Hit
CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGT367760.23631078106252076No Hit
CGCAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTGATCTAGCCATGGGC362640.2330208332730926No Hit
GTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGGCAAGACGGAA353020.22683932980936233No Hit
CAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAG327620.21051810444774596No Hit
CTTGTGGCTGGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTC324130.20827554238034277No Hit
AGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTG291730.18745634152536758No Hit
CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGC267220.17170700161933544No Hit
CGAATGGCGTAATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT260390.16731826267367247No Hit
CCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAG256310.16469658552897187No Hit
GTCAAGTCGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCGAA246980.15870142676425217No Hit
GAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGT238800.15344522111629857No Hit
CTTCGGGCCTCATGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACG235360.1512347874452765No Hit
CGAGCGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAAT220770.14185972138126146No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAG218640.1404910516954251No Hit
GGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGGAAACGATGTGGGAAGGCACAGAC216030.1388139494043299No Hit
CTGCATTCGAAACTGGCAGGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAG213040.13689267130073804No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAGCC207790.1335191896807189No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA199810.12839149761828986No Hit
CCAAAATGCACAGGTTGTGAACTCGAAGAGTAGGGCGGGACACGTGGTAT191450.12301962974336415No Hit
GCACGAATGGCGTAATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGA187870.12071923656247492No Hit
CGGACAGTGTCTGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCCAAAGCGTAACGGAGGAGCACGAAGGTTAGCT185060.11891362068585515No Hit
CCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACC183760.11807828237994566No Hit
CGTGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGG181990.11694093714805351No Hit
GTCCGAATGGGGAAACCCAGTGCAATTCGTTGCACTATCGTTAACTGAAT179570.11538592276320658No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA176760.11358030688658682No Hit
GTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCAA176460.11338753650830002No Hit
CTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCA173640.11157549495240404No Hit
CGGTGATCTAGCCATGGGCAGGTTGAAGGTTGGGTAACACTAACTGGAGG173590.1115433665560229No Hit
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT167060.10734739798864673No Hit
CTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGA159750.10265022643772485No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC156740.10071609697558057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)