FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002798664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18191647
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT2450141.3468489136799984No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG429980.23636122666628262No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC392850.2159507602582658No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT385740.21204237307375193No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA367770.20216421305888355No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG299490.16463050321941713No Hit
AATTTATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTAATGTTT275240.1513002093763143No Hit
CACCACCATCCTTAGGAATTTATTCAAGAGAAGGTAAAGAACACTTGCAC224740.12354021601232697No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT222120.12209999457443298No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA216940.11925253386897844No Hit
CATCACCACCATCCTTAGGAATTTATTCAAGAGAAGGTAAAGAACACTTG215670.11855441126358707No Hit
CTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGCAAA209960.11541560805352039No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT208650.11469549733457338No Hit
AGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA206230.11336521646445757No Hit
CCACCATCCTTAGGAATTTATTCAAGAGAAGGTAAAGAACACTTGCACAC201230.11061670227000336No Hit
ATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGAT198270.1089895818668865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)