FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002798681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17688442
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5509293.114627054208618No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC653730.3695803169097651No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC536010.30302838429749773No Hit
GCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGC486490.2750327021452766No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT413980.23403983233797526No Hit
CCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAAGC342600.19368579776556918No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC323350.18280298513571744No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT318160.17986886578252625No Hit
GCTCATTTTTCTTTTTCTGCAAGCTCAGCAGCAGAATGCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCG271140.15328653591989616No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT219510.12409798443526003No Hit
GTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATTCTGCAATAGCA218680.12362875147511579No Hit
CATGTTTTTCCTTGAGCCTGGATGCTTTGGAGCCAAGCTTTCGTCCCATG214990.12154264349567927No Hit
CTTGTAACAAGGCATTTATTTGGGATGAGGAAAGGAAATTGAGCAGAAGGTACAGGAGTAATAGCAATTCCCTGT207160.11711602412467982No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA205140.1159740354746902No Hit
CTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAAGCTCAGCAGCAGAATGCCCAGAGTCTTCCCTGGT199550.11281377975516443No Hit
CCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTGG192850.10902599561906018No Hit
GTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAG189090.10690031377551511No Hit
CCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATTCTGCAATAGC188990.10684377968393145No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC182920.10341216032480419No Hit
CCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAAG180360.10196488758026287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)