FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002798683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16923055
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCA769790.45487649836273647No Hit
CTCCATTGCATTCAGCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTTGA751680.44417512086322475No Hit
GGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCACATTTTTTTCCAAAT456890.2699808042933146No Hit
GGTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCACATTTTTTTCCAAATGTAATGCAC429600.25385487431199627No Hit
CCCGAGGCTTAGCTTTCATTATCACTGTCTCCCAGGGTGTGCTTGTCAAA397860.2350993954696714No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA388860.22978120676201783No Hit
GTGGATGTTTTGGTACAACTTATAGAAAAGGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTGACCAGGGA368710.21787437315543795No Hit
CCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCT343730.20311344494241731No Hit
GTCTCAATGAAGTCACACAAATGGGGGTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTC295430.1745724988780099No Hit
CTCCCATCTTGCGCAAGTTGGTCACGTGGTCACCCAATTCTTTGATGGCT272620.16109384505339017No Hit
GCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTTGATATCCTGAAGGAAGATTCGGCCACCTCGTTGGTTCTGC237780.14050654565620688No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT217340.12842834819126925No Hit
CTTTCATTATCACTGTCTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGC212510.12557425358482852No Hit
GCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCTATGGGGAAA210880.12461107051888681No Hit
GCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCACATTTTTTTCCAAATG210200.12420925181653075No Hit
CTGGTTTCTTGATATCCTGAAGGAAGATTCGGCCACCTCGTTGGTTCTGC207230.12245424954300509No Hit
GGATGTTTTGGTACAACTTATAGAAAAGGTAAAGGAAACCCCAACATGCA204860.121053793183323No Hit
CACTCCATTGCATTCAGCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTT199240.11773287979032156No Hit
GGCAAAGTTCTTCAAAGCCACATCATCGCGGTCAAAGTAGTAAGACATGG197810.11688787869566103No Hit
GTTTTGGTACAACTTATAGAAAAGGTAAAGGAAACCCCAACATGCATGCA192890.11398060220214376No Hit
GTACAACTTATAGAAAAGGTAAAGGAAACCCCAACATGCATGCACTGCCT188940.11164650826934025No Hit
CGCACGATTTCCCGCTCGGCCGTGGTGGTGAAGCTGTAGCCGCGCTCGGTGAGGATCTTCATGAGGTAGTCAGTC187860.11100832562442184No Hit
CCTGAAGGAAGATTCGGCCACCTCGTTGGTTCTGCAGCTTCATCAGTTTCTCAGCATGTTCCCTCTCCTCATG181740.10739195730321742No Hit
CTCAGCATGTTCCCTCTCCTCATGAGATTGGTGAAGAAAGTATTTGGCAA179450.1060387737320478No Hit
CGCCAAGCCAGATTCGGGCGCTCCCATCTTGCGCAAGTTGGTCACGTGGT169530.10017694795650077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)