FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002798685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17598700
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3029811.7216101189292392No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT328690.18676947729093626No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT286490.16279043338428406No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266770.15158506026013285No Hit
GTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCA231490.1315381249751402No Hit
GTGGATGTTTTGGTACAACTTATAGAAAAGGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTGACCAGGGA220820.1254751771437663No Hit
CTCCATTGCATTCAGCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTTGA205120.11655406365242886No Hit
GGTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCACATTTTTTTCCAAATGTAATGCAC179860.10220073073579299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)