FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10948209
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA236507721.602410038025397No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA6536455.970337248768269No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC3627413.313245116164662No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC2540972.3209001581902573No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2425122.2150837639288765No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2117281.9339053538345858No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1842881.6832707523212243No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC1826851.6686290881001633No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1513891.3827741140126206No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC1206261.1017875161133661No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1105791.0100190816598404No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC1031610.942263707241979No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA1027890.9388658912156317No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC893630.8162339611894511No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC873790.7981122757155987No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG729510.6663281638119989No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG724720.6619530189823742No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA599440.5475233437724837RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC590030.5389283306520729No Hit
CGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG566150.5171165439022949RNA PCR Primer, Index 15 (97% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTGGAATTCTCGGGTG523160.47784984740426495No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA506350.46249573788735676RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA486420.4442918471870605No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC461820.4218224186257314No Hit
CCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCAA458020.41835153128698943No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG435910.3981564473239413No Hit
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAG394890.36068913189362756RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA383210.3500207202840209No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC368480.33656646488937136No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA322800.2948427455120742RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA302560.27635570347624894No Hit
GCATGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG296820.2711128368119388No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT295150.26958747316570225RNA PCR Primer, Index 1 (100% over 30bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG281750.2573480283396124No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG252640.23075920454203974Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA251760.2299554201056995No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC250440.22874974345118912No Hit
CGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCAAG241330.22042874775225793No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA233490.21326775913759044No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC230170.21023529967321594No Hit
CCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGGAA224690.20522991477418817RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGC220970.20183209874784086No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA220010.20095524299910605No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG200080.1827513522988098Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTAATCCTGGAATTCT186400.170256157879339No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTTGGAATTCTCGGGTGCCAAGG180090.1644926581142176Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGG179580.16402682849770223No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT177390.16202650132090096No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG170680.15589764499380676No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATGGAATTCTCGGGTGCCA170570.15579717193926423No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGAA168820.15419873698063308RNA PCR Primer, Index 1 (100% over 23bp)
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAA163850.14965918169812067No Hit
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA163020.14890106683202706No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC162320.1482616928485746No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTTGGAATTCTCGGGTGCCAAGG157780.14411489587018297Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA155620.1421419704355297No Hit
CCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGAAC152870.13963014407196647RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG151120.13803170911333534Illumina Small RNA Adapter 2 (100% over 21bp)
GGCGGAGCGCTGAGAAGACGGTCGAACTTGATGGAATTCTCGGGTGCCAA142210.12989339169539052No Hit
CTGGCGGAGCGCTGAGAAGACGGTCGAACTTGATGGAATTCTCGGGTGCC140100.12796613583098387No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG139170.12711668182439703No Hit
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA138840.12681526266076942No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA129440.11822938345440794No Hit
CGGCTCGAAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGA129280.1180832408296188RNA PCR Primer, Index 19 (100% over 37bp)
GCGGAGCGCTGAGAAGACGGTCGAACTTGATGGAATTCTCGGGTGCCAAG127690.11663094849577679No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC122830.1121918662678069No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA122180.11159816185460107RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTTGGAATTCTCGGGT121860.1113058766050228No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC121270.11076697567611288No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG119290.10895846069434735No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG114530.10461071760687068No Hit
CTGGCGGAGCGCTGAGAAGACGGTCGAACTGGAATTCTCGGGTGCCAAGG114370.10446457498208156Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG114070.10419055756060193No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC110380.10082014327640255No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC109990.10046392062847906RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)