FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002798694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6458975
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1692582.6205086720416166No Hit
GGAGAGGTGTCCGAGCGGCTGAGGGAGCATGATTGGAATTCTCGGGTGCC1314442.035059742451395No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1065311.6493483873215178No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1021021.5807771356910347No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC689241.067104300604972RNA PCR Primer, Index 6 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA679801.0524889785143927RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG372120.5761285652909324Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC318450.4930348855662082RNA PCR Primer, Index 1 (100% over 31bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA269130.41667602057602016RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG254110.39342155682596697No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC231830.35892691951896394RNA PCR Primer, Index 1 (100% over 24bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA220540.3414473658746163RNA PCR Primer, Index 1 (100% over 22bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG218360.33807221734098675RNA PCR Primer, Index 6 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC205330.31789873780282474No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG202850.3140591192875031Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA191390.29631636598686323RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG186910.28938028092692725No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA186270.2883894116326507No Hit
CGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC182140.2819952082180222RNA PCR Primer, Index 6 (100% over 42bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC173080.26796821477091953RNA PCR Primer, Index 6 (100% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA160930.24915718051238778No Hit
AAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAATTC155040.24003808653849878No Hit
AGTTCCAGGGAAAATCGGTCGTCGCCAAACTGGAATTCTCGGGTGCCAAG146790.22726516204196484No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC114580.17739656834095194RNA PCR Primer, Index 5 (97% over 35bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT94300.14599839757856314RNA PCR Primer, Index 6 (100% over 46bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA92120.1426232490449336No Hit
GTGTTTGTAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGAATTCTCGG91700.1419729910705646No Hit
CCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT83550.12935488990126143RNA PCR Primer, Index 6 (100% over 46bp)
AGGGGCGTAGTTCAATTGGTAGAGCACCTGGAATTCTCGGGTGCCAAGGA83410.1291381372431384RNA PCR Primer, Index 1 (100% over 22bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC82060.12704802232552379No Hit
GGAGAGGTGTCCGAGCGGCTGATGGAGCATGATTGGAATTCTCGGGTGCC81110.125577200716832No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG80180.12413734377358637RNA PCR Primer, Index 6 (100% over 45bp)
GGAGAGGTGTCCGAGCGGCTGAAGGAGCATGATTGGAATTCTCGGGTGCC77770.12040610158732616No Hit
CTAAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAAT74940.11602460142669695No Hit
GTCCCCATCGTCTAGAGGCCTAGGACATCGCCCTGGAATTCTCGGGTGCC70730.10950653935028391No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC70220.10871694038140726No Hit
GCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG69910.10823698806699206RNA PCR Primer, Index 6 (100% over 45bp)
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG67960.10521793318599314No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT67640.10472249853885486No Hit
TTAAGTGACGATAGCCTAGGAGATACACCTTGGAATTCTCGGGTGCCAAG67270.10414965222810121No Hit
CAAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACT64630.10006231638921036RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)