FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002798697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5958038
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC2835874.7597380211405165RNA PCR Primer, Index 11 (100% over 50bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC2778654.663699694429609No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA1419572.382613202534123RNA PCR Primer, Index 1 (100% over 23bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG981411.6472033243158233RNA PCR Primer, Index 6 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC582820.9782079268376603RNA PCR Primer, Index 6 (97% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA453890.7618111868370091No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG445730.7481154030907491RNA PCR Primer, Index 2 (97% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA415280.6970079747729034No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA374270.6281765910187213No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG342760.5752900535377586RNA PCR Primer, Index 11 (100% over 45bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA227050.3810818259299454RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT177160.29734620692248015RNA PCR Primer, Index 11 (100% over 37bp)
GTGTTTGTAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGAATTCTCGG146510.2459030976304616No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG136410.22895120843472297No Hit
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT127670.21428194986336108RNA PCR Primer, Index 11 (100% over 46bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA115900.19452712453327758RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA114410.1920263012756884No Hit
TGTAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGAATTCTCGGGTGCC107240.17999213835158487No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC95800.1607911866288869RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG94670.1588945891248092Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTA93560.15703155971814883RNA PCR Primer, Index 11 (100% over 47bp)
GTAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGAATTCTCGGGTGCCA93210.156444118013346No Hit
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTTGGAATTCTCGGGTGCCA89290.14986477091955439No Hit
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATG87580.14699469859037487RNA PCR Primer, Index 11 (100% over 49bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC83880.14078460056817363No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT81050.13603471478362508RNA PCR Primer, Index 1 (100% over 25bp)
CCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT79010.13261076884706005RNA PCR Primer, Index 11 (100% over 46bp)
GCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTA78510.13177156641162743RNA PCR Primer, Index 11 (100% over 47bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAGCTCGTATGC77810.13059668300202182RNA PCR Primer, Index 11 (98% over 50bp)
AGAGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC77190.12955607198208538RNA PCR Primer, Index 11 (100% over 42bp)
GTTTGTAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGAATTCTCGGGT73590.12351381444697064No Hit
AGATTGAGGGTTCGTGTCCCTTCGTGGTCGCCTGGAATTCTCGGGTGCCA71260.1196031310978547No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC69720.11701838759672228No Hit
GCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG68550.11505465389780999RNA PCR Primer, Index 11 (100% over 45bp)
AGATTGAGGGTTCGAGTCCCTTCGTGGTCGCCTGGAATTCTCGGGTGCCA66090.11092577791548157No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA65410.10978446260329322No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG65400.10976767855458457RNA PCR Primer, Index 11 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC64780.10872706753464814No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA63080.1058737792541773RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGGAATTCTCGGGTGC62440.10479960013682356No Hit
CGCGACCTCAGATCAGACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT62010.10407788604235152RNA PCR Primer, Index 1 (100% over 30bp)
GGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC61880.10385969340913906RNA PCR Primer, Index 11 (100% over 44bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA61380.10302049097370644RNA PCR Primer, Index 1 (100% over 23bp)
AGATTGAGGGTTCGGGTCCCTTCGTGGTCGCCTGGAATTCTCGGGTGCCA60250.10112389346962876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)