FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9515691
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2525222.653743170096633No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2493382.620282646840886RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC1727091.8149916805831545RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC1361001.4302692258502299RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG1272281.3370337477330863Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1133171.1908436286970647RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1035581.0882867045598685No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG1013481.0650619066970544No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC791040.8313006380724217No Hit
CTACGGGGATGATTTTACGAACTGAACTTGGAATTCTCGGGTGCCAAGGA745790.7837476017243519RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA704090.739925245575965RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC638630.6711336044854757No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG605250.6360547016501482No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA599260.6297598356230777No Hit
GGTTCGTGCTGAAGGCCTGTATCCTAGGCTACACACTGAGGACTTGGAAT588280.6182209993998334No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC569440.5984221219457421No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA566830.595679283827102RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA561410.5899834284236426No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC554840.5830790428146522RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA504340.5300088033543754No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG467340.49112565761120236No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGT447630.470412500784231No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG444200.4668079280842558Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC441260.46371829434141987No Hit
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC427160.44890066312577825RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA415670.4368258700287767RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC390540.4104168577983459No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC383190.40269277344125615No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTTGGAATTCTCGGGT373010.3919946538827291No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA366990.3856682609807317No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG359540.3778390870405523Illumina Small RNA Adapter 2 (100% over 21bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC356100.3742240053822681RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA339970.3572730556299064RNA PCR Primer, Index 1 (100% over 23bp)
CTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGGT338940.356190632924083No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA333690.350673429811876No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA330330.3471424198200635No Hit
GACGCGGGTGATGCGAACTGGAGTCTGAGCCTTGGAATTCTCGGGTGCCA326180.34278120212184277No Hit
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT298460.31365036968938986RNA PCR Primer, Index 1 (100% over 30bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAATTCTCGGGTGCCAA292980.30789146053607663No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT274050.28799800245720464RNA PCR Primer, Index 1 (100% over 30bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA262320.2756709943607879RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG249450.26214596501714904No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA245890.2584047758591573No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCG245370.25785831002709103No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA242290.25462155086792965RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA239320.2515003902501668RNA PCR Primer, Index 1 (100% over 23bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA238130.25024982421139985RNA PCR Primer, Index 1 (100% over 22bp)
CGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG238040.25015524358661917Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC232090.2439024133927846RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG230000.2417060411062108No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAA229430.24110703048259974No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA225780.23727126069982726No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCTGGAATTCTCGGGTGCCAAG216950.22799185051301057No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA216690.22771861759697745RNA PCR Primer, Index 1 (100% over 22bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC215470.22643652468328362No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA206220.2167157382474904No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG203840.21421460616995652Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCATTCATCTCG200140.21032629159563926RNA PCR Primer, Index 45 (100% over 45bp)
CTACGGGGATGATTTTACGAACTGAACTCTTGGAATTCTCGGGTGCCAAG199160.20929641368136062No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC195990.2059650738974185RNA PCR Primer, Index 1 (100% over 26bp)
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGG195430.20537657223211642No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAG186510.19600258142051902No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA182030.19129456809810239No Hit
CACATGATATTTGTGGAAATTCTGACTTGGAATTCTCGGGTGCCAAGGAA180470.18965517060190373RNA PCR Primer, Index 1 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA178330.1874062535237851No Hit
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGT171840.18058593958126637No Hit
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG171300.1800184558325822Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTG168610.17719154604747045No Hit
TACGGGGATGATTTTACGAACTGAACTTGGAATTCTCGGGTGCCAAGGAA164840.17322966876499038RNA PCR Primer, Index 1 (100% over 23bp)
CACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGG164340.1727042208495421No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA162700.1709807516868717RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA160970.16916270189942068RNA PCR Primer, Index 1 (100% over 22bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTC160410.16857420023411857RNA PCR Primer, Index 1 (100% over 26bp)
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGT159590.1677124656527834No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG156650.16462283190994748No Hit
GAACGCGCCCGATCTCGTCTGATCTCGGAAGTGGAATTCTCGGGTGCCAA153590.16140709066740397No Hit
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA153190.16098673233504535RNA PCR Primer, Index 1 (100% over 32bp)
ATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGGTGC147840.1553644396397487No Hit
CGACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGAACTCCA146900.15437659755870595RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGTGGAATTCTCGGGTGCCAAGGAACT141070.14824987486457894RNA PCR Primer, Index 1 (100% over 25bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA140490.14764035528265892RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA139880.14699930882581203RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA138960.14603248466138719RNA PCR Primer, Index 1 (100% over 28bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG138870.1459379040366065Illumina Small RNA Adapter 2 (100% over 21bp)
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTTGGAATTCTCGGG138840.1459063771616796No Hit
CTCGTACGACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGA135070.14194449987919952RNA PCR Primer, Index 1 (100% over 22bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC134500.14134548925558849No Hit
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG131510.13820331072120776RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA131430.13811923905473603No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGGAATTCTCGGGTGCCAAGG129860.1364693326002284Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC129330.13591235780985322No Hit
ACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGG127290.13376853031482422No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCTGGAATTCTCGGGTGCCA126230.13265458073407385No Hit
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG126060.13247592844282144RNA PCR Primer, Index 1 (100% over 29bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG121270.12744213741282687Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA120440.12656989387318274No Hit
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTTGGAATTCTCGG119850.12594986533295377No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGTGGAATTCTCGGGT118110.12412130658719371No Hit
GACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGGGTGCCAA117340.12331211679740335No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG117220.12318600929769577Illumina Small RNA Adapter 2 (100% over 21bp)
CTTAATGATGACTGTTTTTTTTGATTGCTTGGAATTCTCGGGTGCCAAGG114850.1206953861784709Illumina Small RNA Adapter 2 (100% over 21bp)
GGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTC113670.11945532909801296No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCATTCATCT113310.11907700659889019RNA PCR Primer, Index 45 (100% over 43bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG112230.1179420391015219No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCTGGAATTCTCGGGTG112180.11788949430997706No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG111820.11751117181085431RNA PCR Primer, Index 1 (100% over 29bp)
CTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAA111650.11733251951960189RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC110580.11620806098054255No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCA110450.116071444522526No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC110440.11606093556421704No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC106410.11182582536570387No Hit
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTTGGAATTCTCG106120.11152106557474387No Hit
GCAGCCGACTTAGAACTGGTGCGGACCTGGAATTCTCGGGTGCCAAGGAA104850.11018642786950522RNA PCR Primer, Index 1 (100% over 23bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA100950.1060879341290086RNA PCR Primer, Index 1 (100% over 23bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA100490.10560452204679618RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA99470.1045326082992817RNA PCR Primer, Index 1 (100% over 28bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTTGGAATTC99030.1040702141336872No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCG98140.10313491684418924No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGC98140.10313491684418924No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC97810.10278812121999338RNA PCR Primer, Index 1 (100% over 24bp)
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGGG97300.10225216434623613No Hit
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC96960.10189485976373129RNA PCR Primer, Index 1 (100% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG95550.10041309664216715No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT95240.1000873189345892RNA PCR Primer, Index 3 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)