FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10606545
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC4244704.001962938921204No Hit
GGGTCTGTAGCTCAGGTGGTTAGAGCGCACCTGGAATTCTCGGGTGCCAA2272302.1423564412351053No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1455561.3723224669296175No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1072491.011158676081608No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA932700.8793626953923263No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGC695090.6553406410852921RNA PCR Primer, Index 39 (100% over 50bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG657550.6199474004022988RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA646220.6092653168397437No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG565460.5331236514812316Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGGCGTAGTTCAATTGGTAGAGCACCTGGAATTCTCGGGTGCCAAGGA536690.5059988903078241RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC501740.47304753810029565No Hit
GTCCCCATCGTCTAGAGGCCTAGGACATCGCCCTGGAATTCTCGGGTGCC469330.4424909336640725No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC467490.44075615575100097No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA418510.3945771219563015No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA388760.36652840298136674No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA380960.35917445313247626RNA PCR Primer, Index 1 (100% over 23bp)
CATGTGAGAGTAGGACACCGCCAGGTTGGAATTCTCGGGTGCCAAGGAAC380150.35841077372509145RNA PCR Primer, Index 1 (100% over 24bp)
GCCTCCTTAGCTCAGCTGGCAGAGCAGCGGACTTGGAATTCTCGGGTGCC374860.3534232872250106No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA370030.34886949520319765No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC363960.34314661371822774RNA PCR Primer, Index 1 (100% over 31bp)
TTAAGTGACGATAGCCTAGGAGATACACCTTGGAATTCTCGGGTGCCAAG310390.2926400632816813No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG307610.2900190401304101Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA306790.2892459325821934RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG284250.26799490314706625No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA277570.26169690507134985RNA PCR Primer, Index 1 (100% over 22bp)
GTCCCCATCGTCTAGAGGCCTAGGACATCGCCTGGAATTCTCGGGTGCCA264720.24958174410234435No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC260000.24513166162968242No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA231400.21816717885041734RNA PCR Primer, Index 1 (100% over 23bp)
CCCGTGAAAGTAGGACATCGTCAGACATGGAATTCTCGGGTGCCAAGGAA228800.2157158622341205RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGAAAGTAGGACATCGTCAGACATGGAATTCTCGGGTGCCAAGGAAC225780.21286856370288346RNA PCR Primer, Index 1 (100% over 24bp)
GAACATAAGAGTTTGATCCTGGCTCATGGAATTCTCGGGTGCCAAGGAAC221790.20910673551095102RNA PCR Primer, Index 1 (100% over 24bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA218110.20563717968480782RNA PCR Primer, Index 1 (100% over 32bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC217680.20523176962903567RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA212380.20023485498812288No Hit
CGCTTAGCAGGGGTTGTAGGACCACGCTGGAATTCTCGGGTGCCAAGGAA210330.19830208611758118RNA PCR Primer, Index 1 (100% over 23bp)
ATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAAC206750.1949268116997571RNA PCR Primer, Index 1 (100% over 24bp)
GGAGTAATGGCTGAGAGGCTGAAGGCACTGGAATTCTCGGGTGCCAAGGA205170.19343716544831518RNA PCR Primer, Index 1 (100% over 22bp)
ACTGAACAAGACGAACCAATGTGCATGGAATTCTCGGGTGCCAAGGAACT201320.18980733122802948RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC196070.1848575572912763No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC196000.1847915603054529No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG195800.1846029974888147No Hit
GGGTCTGTAGCTCAGGTGGTTAGAGCGCACCCTGGAATTCTCGGGTGCCA188230.17746589487905817No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC187290.17657964964085854No Hit
AGTAGGTGATTGGTTCGACTCCGATAAGCGGCACCATGGAATTCTCGGGT181140.17078134302923337No Hit
AGAGAAAGAAGAGGTCTGATGCATTCACACTTGGAATTCTCGGGTGCCAA178420.16821688872295362No Hit
ATGCGAAAGTAGGTCACTGCCAAACACCTGGAATTCTCGGGTGCCAAGGA174490.16451162937601263RNA PCR Primer, Index 1 (100% over 22bp)
AATACTAATAGCTCGAGGACTTATCCTGGAATTCTCGGGTGCCAAGGAAC161840.1525850312236454RNA PCR Primer, Index 1 (100% over 24bp)
GCGGTTGTAGCTCAGTTGGTAGAGCACCTGGAATTCTCGGGTGCCAAGGA157410.14840836483510889RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC156260.14732412863943914No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCC155970.1470507125553137No Hit
CATGCGAAAGTAGGTCACTGCCAAACACCTGGAATTCTCGGGTGCCAAGG155410.1465227366687267Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG154420.14558935072636756Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA152320.14360944115166624No Hit
AGAGGGTCGGGCGTTCGAATCGCTCCGGGTGCGCCATGGAATTCTCGGGT151670.14299661199759203No Hit
GGGTCTGTAGCTCAGCTGGTTAGAGCACCTGGAATTCTCGGGTGCCAAGG151410.14275148033596238Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA149340.14079985518375682No Hit
GTCCCCATCGTCTAGAGGCCTAGGACATCGCCCTTTCTGGAATTCTCGGG148420.13993246622722103No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA144920.13663261693605222No Hit
GAACATAAGAGTTTGATCCTGGCTCAGATTGAACGCTTGGAATTCTCGGG144000.13576522797951643No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG143510.1353032490787528Illumina Small RNA Adapter 2 (100% over 21bp)
GCGCCCATCGTCTAGCGGTTAGGACTGGAATTCTCGGGTGCCAAGGAACT141900.13378531840481514RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC139690.13170169928096284No Hit
CTAACCCATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCC139000.13105115756356098No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA138300.13039118770532723RNA PCR Primer, Index 1 (100% over 28bp)
CGCCCGTGAAAGTAGGACATCGTCAGACATGGAATTCTCGGGTGCCAAGG137280.12942951734047234Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC132330.12476258762867645No Hit
CGGGAAGTAGCTCAGCTTGGTAGAGCACTTGGTTTGGAATTCTCGGGTGC131830.12429118058708091No Hit
GCGGTTGTAGCTCAGTTGGTAGAGCACTGGAATTCTCGGGTGCCAAGGAA131200.1236972077146705RNA PCR Primer, Index 1 (100% over 23bp)
ATACTAATAGCTCGAGGACTTATCCTGGAATTCTCGGGTGCCAAGGAACT130450.12299009715227721RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC129180.12179272326662452No Hit
GTCCCCATCGTCTAGAGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG127760.12045392726849319RNA PCR Primer, Index 1 (100% over 29bp)
ATATGTCGCAGGTTCGAGTCCCGCAGGAGGAGCCATGGAATTCTCGGGTG124890.11774805084973476No Hit
TGAACATAAGAGTTTGATCCTGGCTTGGAATTCTCGGGTGCCAAGGAACT124340.11722950310397967RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA123480.11641868299243534RNA PCR Primer, Index 1 (100% over 22bp)
GGTGGCTGTAGCTCAGTTGGTAGAGCCCTGGAATTCTCGGGTGCCAAGGA123430.11637154228827577RNA PCR Primer, Index 1 (100% over 22bp)
AAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGAATTCTCGGGTGCCAAGG122540.1155324377542357Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGAAGTAGCTCAGCTTGGTAGAGTATGGAATTCTCGGGTGCCAAGGAA122460.11545701262758042RNA PCR Primer, Index 1 (100% over 23bp)
AGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATGGAATTCTCGGGT120990.11407107592528952No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGGAATTCTCGGGTGC119060.11225144474473073No Hit
CGCGCCCGTGAAAGTAGGACATCGTCAGACATGGAATTCTCGGGTGCCAA113640.10714139241383505No Hit
GTCCCCATCGTCTAGAGGCCTAGGACTGGAATTCTCGGGTGCCAAGGAAC113230.1067548386397267RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA112510.10607601249982912No Hit
TTCCGGCGACCATAGTGCAGTAGTTCTACCTTGGAATTCTCGGGTGCCAA112120.10570831500738458No Hit
GCACCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTGGAATTCTCGGGTG110110.10381325870017051No Hit
GAGAAAGCTCACAAGAACTGCTAACTTGGAATTCTCGGGTGCCAAGGAAC109210.10296472602529852RNA PCR Primer, Index 1 (100% over 24bp)
TCCACAGTAGCTCAGTTGGTAGTAGCATCTGACTTGGAATTCTCGGGTGC106830.10072082850730375No Hit
CTGTGAGACTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACT106790.1006831159439761RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC106470.10038141543735496No Hit
CGCTTAGCAGGGGTTGTAGGACCACGCTTGGAATTCTCGGGTGCCAAGGA106310.10023056518404438RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)