FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10289840
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC7708117.490991113564448No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC5085264.942020478452531No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2738822.661674039635213No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC2565112.4928570317905816No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2008001.9514394781648694No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1487461.4455618357525482RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1120931.0893561027187983No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1117771.0862851123049533No Hit
GCGGGTGTAGTTCAATGGTAGAACACCTGGAATTCTCGGGTGCCAAGGAA1076801.0464691384900056RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1055341.0256136149833233No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA806320.7836079083834151No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC795010.7726164838325961No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC728340.7078244170949208No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA691590.6721095760478297No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA673020.6540626482044424No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG630030.6122835729224166No Hit
CGACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAAC592500.5758107025959587RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC589070.5724773174315636No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT509200.4948570628892189No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC484160.4705223793567247No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC443820.4313186599597273No Hit
GACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAACT398950.38771253974794556RNA PCR Primer, Index 1 (100% over 25bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA396880.3857008466603951RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA357870.3477896643679591No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC352930.3429888122653025No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC346090.33634147858470104No Hit
CAAACGAGAACTTTGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT340320.3307340055822054RNA PCR Primer, Index 1 (100% over 30bp)
GGGACTGTAGCTCAACTGGTCAGAGCAACCGACTTGGAATTCTCGGGTGC334000.32459202475451515No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA333400.32400892530884834No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG312260.3034643881731883Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTCCGTAGCTCAGCCGGTTAGAGCATCTGACTTGGAATTCTCGGGTGC307510.2988481842283262No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC304530.29595212364818113No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA300840.29236606205733034No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG292540.2842998530589397No Hit
CGCGGGGTAGAGCAGTCTGGCAGCTCGTCGGGCTGGAATTCTCGGGTGCC284320.2763113906533046No Hit
TGCAATGTTGGGGTTGTAGGACTACGTTGGAATTCTCGGGTGCCAAGGAA272170.26450362687855206RNA PCR Primer, Index 1 (100% over 23bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA251160.24408542795611982RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA242960.23611640219867364No Hit
AAACGAGAACTTTGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC238880.23215132596813942RNA PCR Primer, Index 1 (100% over 31bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA225770.21941060308032No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG224540.21821524921670307No Hit
GCGGCCTTCGTCTAGTGGTTAGGACTCCAGGTTTGGAATTCTCGGGTGCC223510.21721426183497508No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA219890.2136962285127854No Hit
ATGTGGAGGTCGGCAGTTCAAGTCTGCCTGGGACTACTGGAATTCTCGGG212260.2062811472287227No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA211920.20595072420951152No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA211080.205134384985578No Hit
GGCATCTTGGCCGATCGGTTAGGTAACGGTTTGCTGGAATTCTCGGGTGC200590.19493986301050356No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC194400.18892422039604115No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC191220.18583379333400712No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC190390.18502717243416808No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG186570.1813147726300895Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTTGGAATTCTCGGGTGCC186410.18115927944457832No Hit
TGGGCTGTCGCCAAGCGGTAAGGCACTGGACTTGGAATTCTCGGGTGCCA185330.18010970044237812No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC183260.17809800735482767RNA PCR Primer, Index 1 (100% over 27bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCC180590.17550321482161044RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGACTGGAATTCTCGGGT180310.17523110174696593No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA179330.17427870598571016RNA PCR Primer, Index 1 (100% over 28bp)
AGGGGCGTAGTTCAATTGGTAGAGCACCTGGAATTCTCGGGTGCCAAGGA178940.17389969134602676RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA169560.16478390334543588No Hit
GGTACCATAGCTCAGTTGGTAGAGCAAAGGACTGGAATTCTCGGGTGCCA168040.16330671808308No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCG166460.16177122287615744No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA165520.16085770041127948RNA PCR Primer, Index 1 (100% over 28bp)
AATCCTGTGTGAATCTGCCGGGCCCTGGAATTCTCGGGTGCCAAGGAACT156700.1522861385599776RNA PCR Primer, Index 1 (100% over 25bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA155920.15152810928061078No Hit
GTCCCCATCGTCTAGAGGCCTAGGACATCGCCCTGGAATTCTCGGGTGCC154450.15009951563872714No Hit
GGTGACCGTAGTTCAGTTGGTAGAGCGCCAGTTTGGAATTCTCGGGTGCC150330.14609556611181515No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA149770.14555133996252614RNA PCR Primer, Index 1 (100% over 23bp)
GCTTCAATAGCTCAGTTGGTTAGAGCACCTGACTGTTGGAATTCTCGGGT147060.1429176741329311No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA145360.1412655590368752No Hit
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA145270.1411780941200252No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTTGGAATTCTCGGGTGCC144920.14083795277671957No Hit
CGGGATGTAGCACAGTTGGTAGTGTACCTGGAATTCTCGGGTGCCAAGGA140700.1367368200088631RNA PCR Primer, Index 1 (100% over 22bp)
CTACGGGGATGATTTTACGAACTGAACTTGGAATTCTCGGGTGCCAAGGA139610.13567752268256844RNA PCR Primer, Index 1 (100% over 22bp)
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA138900.13498752167186273No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC136830.13297582858431228RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA136070.13223723595313436RNA PCR Primer, Index 1 (100% over 23bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC135820.1319942778507732No Hit
GGTGACCGTAGTTCAGTTGGTAGAGCGCCTGGAATTCTCGGGTGCCAAGG134840.13104188208951745Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC131200.12750441211913888RNA PCR Primer, Index 1 (100% over 26bp)
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT129730.12607581847725524RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG126890.1233158144344324No Hit
CGCGGAGTGGAGCAGTTGGTAGCTCGCCAGGCTGGAATTCTCGGGTGCCA125560.12202327732987102No Hit
GCATGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA122540.11908834345334815No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA117490.11418058978565264No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGATGGAATTCTCGGGTGCCAA117450.11414171648927486No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG114930.11169269881747433No Hit
GGTGCGTTAGTTCAGTTGGTTAGAATACATGCCTTGGAATTCTCGGGTGC114880.1116441071970021No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC113190.11000171042504063RNA PCR Primer, Index 1 (100% over 27bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCTGGAATTCTCGGGTGCCAA112930.109749033998585No Hit
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAG107890.10485099865498394RNA PCR Primer, Index 1 (100% over 29bp)
CGGAATGGAGGACCGCACCGATAAGCGTTTGGAATTCTCGGGTGCCAAGG105570.10259634746507235Illumina Small RNA Adapter 2 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG104980.1020229663435Illumina Small RNA Adapter 2 (100% over 21bp)
GACCCAGTGGCCTAATGGATAAGGCATCAGCCTTGGAATTCTCGGGTGCC104440.10149817684239988No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)