FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002798704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11098738
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGTGTAGCGCAGTCCGGTTAGCGCACCTGGAATTCTCGGGTGCCAAG1032710.9304751585270326No Hit
TAGAGGTCGGCAGTTCAACTCTGCCTGGGACTACCTGGAATTCTCGGGTG680340.6129886118583933No Hit
GCGGAAATAGCTCAGTTGGTAGAGCATAACTGGAATTCTCGGGTGCCAAG638860.5756149933442883No Hit
CGCGGAGTGGAGCAGTTGGTAGCTCGTTGGGCTCTGGAATTCTCGGGTGC536790.4836495825020827No Hit
TATGCGAAACCGAAAGGTGAAGTATATGGGCTGGAATTCTCGGGTGCCAA505260.45524094721399855No Hit
TGAAGAGTCGTTCGAGACCAGGACGTTGATAGGTTGGAATTCTCGGGTGC394640.35557195782079004No Hit
AACCAAAGAGTGTGAGAGTCACGTAGATGAAAACGGCGTGGAATTCTCGG382970.34505724885117567No Hit
ACTGGACGATAACTGACGCTGAGGCTCGAAAGCGTTGGAATTCTCGGGTG371190.3344434295142385No Hit
GCGGGTGTAGTTCAATGGTAGAACACCTGGAATTCTCGGGTGCCAAGGAA367740.33133496799365836RNA PCR Primer, Index 1 (100% over 23bp)
TCTTTGATCCGCAGATGTCTGAATGGGGAAACCCGGCTGTGGAATTCTCG350180.31551334935557535No Hit
AACCAAAGAGTGTGAGAGTCACGTAGATGAAAACGGCGATGGAATTCTCG330340.2976374431038916No Hit
CCATTGATCCGGAGATATCTGAATGGGGCAACCCAATGGTGGAATTCTCG318830.28726689466856503No Hit
ACGGGAGTAGCTCAGTTGGTAGAGCACCTGGAATTCTCGGGTGCCAAGGA314790.2836268411777988RNA PCR Primer, Index 1 (100% over 22bp)
GGCAACACATGGAGCTGACTGTTACTAATAGGTCGAGGGCTTGATTGGAA275640.24835256044425952No Hit
TATGCGAAACCGAAAGGTGAGGTATATGGGCTGGAATTCTCGGGTGCCAA266750.24034264075789516No Hit
GGCAACATGTGGAGCTGACTGTTACTAATAGGTCGAGGGCTTAATGGAAT254310.22913415921702088No Hit
TTGAAAGGGGGGTTCGATTCCCCCAGGGGCTGGAATTCTCGGGTGCCAAG249430.22473726292124382No Hit
CGGGGTGTAGCGCAGTCCGGTTAGCGCGCCTGGAATTCTCGGGTGCCAAG243620.2195024335199191No Hit
TATGTTACGGCATAACCGGGGTAGTAGGACTACTGGAATTCTCGGGTGCC240500.21669130310130758No Hit
CGGCATCGGATTGAGGGAAAAGGATTTGGAATTCTCGGGTGCCAAGGAAC236850.21340264091286773RNA PCR Primer, Index 1 (100% over 24bp)
CGGGGTGTGGCTCAGCTTGGCTAGAGCGCCTGGAATTCTCGGGTGCCAAG222220.20022096205892956No Hit
GAGAGACGTAAGATAGGAGAACGGACTGGGAAGTCCTGGAATTCTCGGGT218660.19701339017102665No Hit
AAGAGAGAATGCCGGAATGAGTAGCGAGAGGAATGGAATTCTCGGGTGCC213180.19207589187167046No Hit
CGCGGAGTGGAGCAGTTGGTAGCTCGTTGGGCTCATAACCCTGGAATTCT212330.19131003903326665No Hit
GGTGGATTCGTCTAACGGTTAGGACACATGCCTTGGAATTCTCGGGTGCC211580.19063428652879275No Hit
AACCGAAGAAGGTGAGAGTCCTGTACACGAAAAACCTGAACTGGAATTCT201220.18129989193365947No Hit
TATGCAAAACCGAAAGGTGAGGTATATGGGCTGGAATTCTCGGGTGCCAA198700.17902936351862705No Hit
GTACGAAAGTAGGACTTAGTGATCCGGTTGGAATTCTCGGGTGCCAAGGA193690.1745153367887412RNA PCR Primer, Index 1 (100% over 22bp)
GAAGAGAGAATGCCGGAATGAGTAGCGAGAGGAATGGAATTCTCGGGTGC182020.16400062781912683No Hit
AACCAAAGAGTGTGAGAGTCACGTAGATGAAAACGGCGAGATGGAATTCT166950.15042250749589728No Hit
ACTGGACGATAACTGACGCTGAGGCTCGAAATGGAATTCTCGGGTGCCAA162750.14663829347084326No Hit
ATGTTACGGCATAACCGGGGTAGTAGGACTACTGGAATTCTCGGGTGCCA161740.1457282800981517No Hit
CGATTGATAGGAGAATGGTCTGGGAAGTCCTGGAATTCTCGGGTGCCAAG155060.1397095777916372No Hit
TGTGCTAAATCGAAAGATGACGTATACGAGCTGACGCCTGGAATTCTCGG153580.13837609284947533No Hit
GGAGCTGACTGTTACTAATAGGTCGAGGGCTGGAATTCTCGGGTGCCAAG151800.13677230690552383No Hit
CGGGATGTAGCACAGTTGGCTAGCGCGCCACTGGAATTCTCGGGTGCCAA150910.13597041393354814No Hit
GGCGGTATCGTCTAGTGGCCTAGGACGCGGCCTGGAATTCTCGGGTGCCA146590.13207807950777828No Hit
GCACTGATCCACGGATGTCCGAATGAGGGAACTCACCCGCTGGAATTCTC141550.12753702267771344No Hit
TACTGGACGATAACTGACGCTGAGGCTCGAAAGCGTTGGAATTCTCGGGT137020.1234554775506909No Hit
GGCCCATTCGTCTATCGGTTAGGACGCAAGTGGAATTCTCGGGTGCCAAG134890.12153634043798493No Hit
GCGGATGTAGCTCATCTGGTAGAGCGCCTGGAATTCTCGGGTGCCAAGGA134160.12087860800029697RNA PCR Primer, Index 1 (100% over 22bp)
AACCAAAGAGTGTGAGAGTCACGTAGATGAAAACGGCGAGTGGAATTCTC132350.11924779195616655No Hit
GAAGAGAGAATGCCGGAATGAGTAGCGAGAGAGATGGAATTCTCGGGTGC129320.11651775183809186No Hit
GCACTGATCCACGGATGTCCGAATGAGGGAACTCACCCTGGAATTCTCGG128960.11619339063594439No Hit
CCATTGATCCGGAGATATCTGAATGGGGCAACCCAATGGATGGAATTCTC124760.11240917661089035No Hit
GGCAACATGTGGAGCTGACTGTTACTAATAGGTCGAGGGCTTAATTGGAA120560.10862496258583633No Hit
CGCCATAGAAGGTAATAGCCCTGTAGTTGAAACGAGAATGGAATTCTCGG118780.10702117664188487No Hit
GGTGGATGTAGCTCAGTCGGTTAGAGCGTCTGGAATTCTCGGGTGCCAAG115530.10409291578916449No Hit
GGTTCTGCTGAGGACTCTGACAAGACTGCCTGGAATTCTCGGGTGCCAAG114370.10304775191557815No Hit
AGCCAGAGAGGGTGAAAGCCCCGTACACGAAATCCGGACTGGAATTCTCG113230.10202060810877778No Hit
ACAAGCCAAAGGCGTGACGCGGACCGAACTGGAATTCTCGGGTGCCAAGG112990.1018043673073461Illumina Small RNA Adapter 2 (100% over 21bp)
TCTTCCTTAGCTCAGTCGGTTAGAGCATCTGACTGTTAATCTGGAATTCT111510.10047088236518423No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)