FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002798705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5913109
Sequences flagged as poor quality0
Sequence length76
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCTGTGGCGCAGTTTGGTAGCGCACTTGACTTGGAATTCTCGGGTGC149079725.21172872003543No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC2289163.871330631652486No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGTGGAATTCTCGGGTGCCA1565462.6474397816783015No Hit
GCCCGAGTGGCGGAATGGTAGACGCGGCGCACTTGGAATTCTCGGGTGCC1308442.2127784216391073No Hit
GCCCGAGTGGCGGAATGGTAGACGCGGCGCACTCTGGAATTCTCGGGTGC1010651.709168560904255No Hit
CGGGCTGTGGCGCAGTTTGGTAGCGCACTTGACTGTGGAATTCTCGGGTG476370.8056168083490427No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA461680.7807737012796483No Hit
TCCCCTGTAGCTCAATTGGCAGTGGAATTCTCGGGTGCCAAGGAACTCCA453290.7665848879159847RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA450210.7613761221042941No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA349750.591482416441165No Hit
CTTAAGCCGCGGTTCGACTCCGCGCAGGACCTTGGAATTCTCGGGTGCCA317080.5362322933671609No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACCTTGGAATTCTCGGGTGCCA300520.5082267213406687No Hit
CGGGAAGTAGCTCAGCTTGGTAGAGCACTTGGAATTCTCGGGTGCCAAGG286830.4850747720023426Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGCTGTGGCGCAGTTTGGTAGCGCATGGAATTCTCGGGTGCCAAGGAA276150.4670132074345323RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGGTGGAGCAGTTCGGTAGCTCGCCGGGCTGGAATTCTCGGGTGCC251270.4249372030855511No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA219020.37039736625859593No Hit
TCCCCTGTAGCTCAATTGGCAGATGGAATTCTCGGGTGCCAAGGAACTCC215990.36527315833345875RNA PCR Primer, Index 1 (100% over 27bp)
GCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCTGGAATTCTCGGGTGC183200.3098200963317267No Hit
CGGGAAGTAGCTCAGCTTGGTAGATGGAATTCTCGGGTGCCAAGGAACTC174680.2954114324630241RNA PCR Primer, Index 1 (100% over 26bp)
CGGGCTGTGGCGCAGTTTGGTAGCTGGAATTCTCGGGTGCCAAGGAACTC164670.2784829435750297RNA PCR Primer, Index 1 (100% over 26bp)
CGCTAAGGAGTGTGTAACAACTCACCTTGGAATTCTCGGGTGCCAAGGAA162310.2744918113297083RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGGTGGAGCAGTTCGGTAGCTCGCCGGGCTTGGAATTCTCGGGTGC152670.2581890508022091No Hit
CGGGCTGTAGCGCAGTTTGGTAGCGCACTTGACTTGGAATTCTCGGGTGC148820.25167809353759585No Hit
GCCCGAGTGGCGGAATGGTAGACGCGGCGCACTGGAATTCTCGGGTGCCA148470.2510861883317219No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTTGGAATTCTCGGGTGCCAAGG147930.25017296315694504Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGCTGTGGCGCAGTTTGGTAGCGCACTTGGAATTCTCGGGTGCCAAGG145020.24525169415953604Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGACGTGGCTCAGTTGGTAGAGCATCACCTGGAATTCTCGGGTGCCAA140530.23765839594703903No Hit
GCGGGCGTAGCTCAATGGTAGAGCGCCTGGAATTCTCGGGTGCCAAGGAA123940.20960208918861467RNA PCR Primer, Index 1 (100% over 23bp)
GGTGGCTATAGTATAGTGGTCAGTACGTATCGTTTGGAATTCTCGGGTGC123920.20956826603399326No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA121130.20484993596431253No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATCTGACTGTTAATCTGGAATTCTC117060.1979669239988642No Hit
CGGGCTGTGGCGCAGTTTGGTAGCGTGGAATTCTCGGGTGCCAAGGAACT113940.1926905118779309RNA PCR Primer, Index 1 (100% over 25bp)
CGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCC111170.18800600496287148RNA PCR Primer, Index 6 (97% over 36bp)
GGGCTGTGGCGCAGTTTGGTAGCGCACTTGACTTGGAATTCTCGGGTGCC109870.18580749991248258No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG109090.18448839688224924Illumina Small RNA Adapter 2 (100% over 21bp)
GTCCGGGTGGCGGAATTGGTAGACGCGTGGAATTCTCGGGTGCCAAGGAA100810.1704856108690031RNA PCR Primer, Index 1 (100% over 23bp)
TCCCCTGTAGCTCAATTGGCAGAGCTGGAATTCTCGGGTGCCAAGGAACT98400.1664099207371283RNA PCR Primer, Index 1 (100% over 25bp)
TCCCCTGTAGCTCAATTGGCAGAGTGGAATTCTCGGGTGCCAAGGAACTC97740.16529375663462317RNA PCR Primer, Index 1 (100% over 26bp)
GCGGAAGTAGTTCAGTGGTAGAACACTGGAATTCTCGGGTGCCAAGGAAC96930.16392391887245777RNA PCR Primer, Index 1 (100% over 24bp)
TTCTGCGTAGTTTAGTGGTTATGACGGGGCCTTTGGAATTCTCGGGTGCC94920.16052469183301035No Hit
GCCAACTTAGCTCAGTCGGTAGAGCGACGCTCTGGAATTCTCGGGTGCCA89180.15081744645667786No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGTTGGAATTCTCGGGTGCC82160.13894551918457787No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATCTGACTGTTGGAATTCTCGGGTG78730.13314484816701333No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC78400.13258676611576078No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATCTGACTTGGAATTCTCGGGTGCC77920.13177501040484793No Hit
CGCGGGGTGGAGCAGTTCGGTAGCTTGGAATTCTCGGGTGCCAAGGAACT77580.13120001677628468RNA PCR Primer, Index 1 (100% over 25bp)
GCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCTGGAATTCTCGGGTG77060.13032061475612913No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA76910.13006694109646888No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA76380.12917062749900263No Hit
CGCTAAGGAGTGTGTAACAACTCACCTGCTGGAATTCTCGGGTGCCAAGG75440.12758093923179836Illumina Small RNA Adapter 2 (100% over 21bp)
GTCCGGGTGGCGGAATTGGTAGACGCTGGAATTCTCGGGTGCCAAGGAAC75240.12724270768558468RNA PCR Primer, Index 1 (100% over 24bp)
GTCCGGGTGGCGGAATTGGTAGACGCGCTAGCTTTGGAATTCTCGGGTGC72260.12220305764700093No Hit
GCGGAAGTAGTTCAGTGGTAGAACACCACCTTGGAATTCTCGGGTGCCAA70110.11856706852520392No Hit
GCGGCAATCGTCTAGTGGTTTAGGATCTCACCTTGGAATTCTCGGGTGCC68350.11559063091852358No Hit
ATTGACCCCGGTTCGAACCCGGGACGGACCTTGGAATTCTCGGGTGCCAA66940.11320609851771717No Hit
TTCTGCGTAGTTTAGTGGTTATGACGGGGCCTTGTGGAATTCTCGGGTGC66840.11303698274461031No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA66510.11247890069335775No Hit
GGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAAGGAA66340.11219140387907613RNA PCR Primer, Index 1 (100% over 23bp)
ACGAGGTCGGTTGTTCGATCCAGCCAGTGGGCACCTGGAATTCTCGGGTG65500.11077083138497869No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGGCCTTGGAATTCTCGGGTGC64420.10894438103542486No Hit
CGGGCTGTGGCGCAGTTTGGTAGCGCACTGGAATTCTCGGGTGCCAAGGA63240.10694881491276416RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC63230.10693190333545348No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC61390.10382017311028767No Hit
CGGGAAGTAGCTCAGCTTGGTAGAGTATGGAATTCTCGGGTGCCAAGGAA61340.10373561522373424RNA PCR Primer, Index 1 (100% over 23bp)
GGACCGGCGTCGGCGTGTGGACGGCCTGGAATTCTCGGGTGCCAAGGAAC59290.10026874187504407RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)