FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002798707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5564214
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG4921408.844735303135359No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA3881106.975109152883048No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1313562.360728757017613Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC587431.0557286258220837No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG502650.9033620921122013No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC408740.7345871312641822RNA PCR Primer, Index 16 (100% over 50bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG362460.6514127601849965No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA317510.5706286638148712No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA283150.508876905165761No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA143960.25872477226792495No Hit
TTAAATTTTCAACGATGGATATCTAGGCTCTGGAATTCTCGGGTGCCAAG137600.24729458644113977No Hit
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA123540.22202596808821515RNA PCR Primer, Index 1 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA102670.1845184243452894No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG92950.16704964977982514No Hit
TCCGGATTAGTTTAGGTGGTTATGATACATCGCTTGGAATTCTCGGGTGC79050.14206858327159955No Hit
TTAAATTTTCAACGATGGATATCTAGGCTTGGAATTCTCGGGTGCCAAGG77270.13886956899932318Illumina Small RNA Adapter 2 (100% over 21bp)
GTGTGAAAGTAGGTCATCGTCAGGCTTGGAATTCTCGGGTGCCAAGGAAC69310.12456386472554794RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA59320.10660984642215415No Hit
ATACGATGAAGAGTTTGATCCTGGCTCTGGAATTCTCGGGTGCCAAGGAA56280.10114636137287314RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)