FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002798708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6063400
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGC645871.0651944453606887RNA PCR Primer, Index 41 (100% over 50bp)
TGTATGGTGACTCTGGATAACTTTGTGCTGATCGCATGGCCTTGGAATTC253130.41747204538707655No Hit
ATTGAGACTTAGCCCTTCGACCGGGGATTTGTTGGAATTCTCGGGTGCCA211970.349589339314576No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA157900.26041494870864534No Hit
GCCTAGGACCATATCACGTTGAATGCACCGGTTTGGAATTCTCGGGTGCC141280.2330045848863674No Hit
CCGTGACCACGCCTGTCTGAGGGTCGGTTGGAATTCTCGGGTGCCAAGGA125860.20757330870468713RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC122640.20226275686908335No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG114820.18936570241118844RNA PCR Primer, Index 6 (100% over 34bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT110440.1821420325230069RNA PCR Primer, Index 1 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA104380.1721476399379886No Hit
CCCGTCAGTATGTGTGTCTCAGTGTTGAACTGGAATTCTCGGGTGCCAAG98820.16297786720321933No Hit
GTATGGTGACTCTGGATAACTTTGTGCTGATCGCATGGCCTTGGAATTCT98510.1624666028960649No Hit
TGTATGGTGACTCTGGATAACTTTGTGCTGATCGCATTGGAATTCTCGGG94960.15661180195929678No Hit
TGAATGGTTTAGTGAGATCTTCGGATTGGTCTGGAATTCTCGGGTGCCAA94850.15643038559224198No Hit
GTGTGAAAGTAGGTCATCGTCAGGCTTGGAATTCTCGGGTGCCAAGGAAC90180.148728436190916RNA PCR Primer, Index 1 (100% over 24bp)
GCCTAGGACCATATCACGTTGAATGCACCTGGAATTCTCGGGTGCCAAGG79580.13124649536563643Illumina Small RNA Adapter 2 (100% over 21bp)
GTATGGTGACTCTGGATAACTTTGTGCTGATCGCATTGGAATTCTCGGGT77050.12707391892337633No Hit
CCCGTGTGAAAGTAGGTCATCGTCAGGCTTGGAATTCTCGGGTGCCAAGG70650.11651878484018868Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA65860.10861892667480293RNA PCR Primer, Index 1 (100% over 23bp)
TGTATGGTGACTCTGGATAACTTTGTGCTGATCGCATGTGGAATTCTCGG63210.10424844146848303No Hit
CACCCGGCCCGGACACTGTAAGGATTGACAGATTGGAATTCTCGGGTGCC62830.10362173038229376No Hit
GAATGGTTTAGTGAGATCTTCGGATTGGTCTGGAATTCTCGGGTGCCAAG60710.10012534221723786No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)