FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10753213
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTCGAAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTC3634503.3799200294832814RNA PCR Primer, Index 13 (97% over 37bp)
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG2299742.138653814445971No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG2035621.893034202893591Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1988671.849372833961347RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1803691.6773498302321364No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG1182431.0996062293195532No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1179201.0966024759297524No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGT1130891.0516763687281188No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA773230.7190688029707958RNA PCR Primer, Index 1 (100% over 28bp)
CTGAGAAGACGGTCGAACTTGATGGAATTCTCGGGTGCCAAGGAACTCCA718890.6685350694717942RNA PCR Primer, Index 1 (100% over 28bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA707710.658138176933722RNA PCR Primer, Index 1 (100% over 32bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC678460.6309370045957428No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA636960.5923438882871567No Hit
CGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCG636560.5919719064432184RNA PCR Primer, Index 27 (100% over 45bp)
GAACGCGCCCGATCTCGTCTGATCTCGGAAGTGGAATTCTCGGGTGCCAA631870.5876104193230433No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA596220.554457537482053No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA595130.5534438869573215No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA575600.5352818734270399RNA PCR Primer, Index 1 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC558360.5192494559533044No Hit
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAG543150.5051048463375551RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC537140.499515819132384No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCG516150.479996071871728No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA510650.474881321517578RNA PCR Primer, Index 1 (100% over 23bp)
CTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAA502910.46768347283737427RNA PCR Primer, Index 1 (100% over 23bp)
GGCTCGAAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCC470130.43719956072664046RNA PCR Primer, Index 27 (100% over 38bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT469510.43662298886853634RNA PCR Primer, Index 1 (100% over 30bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTG469460.43657649113804403No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA461980.4296204306564001No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCT439890.40907773332491415RNA PCR Primer, Index 27 (100% over 43bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA435620.4051068271408741No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA429500.39941550492861994RNA PCR Primer, Index 1 (100% over 22bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA426660.3967744338366589RNA PCR Primer, Index 1 (100% over 23bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA377290.3508625747485891RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA377260.35083467611029373No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG356030.3310917397432749Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC355260.3303756746936939RNA PCR Primer, Index 1 (100% over 24bp)
CTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTA328090.30510880794419304RNA PCR Primer, Index 27 (100% over 40bp)
GTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCTGGAATTC321350.2988409138738347No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAATTCTCGGGTGCCAA305280.2838965432936184No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA301640.2805115085137809No Hit
GCAGCCGACTTAGAACTGGTGCGGACCTGGAATTCTCGGGTGCCAAGGAA293410.2728579820747529RNA PCR Primer, Index 1 (100% over 23bp)
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGTGGAATTCTCGGGT276160.25681626505491895No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTTGGAATTCTCGGGT269240.2503809791547884No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA267110.2484001758358176No Hit
AAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTGGAATTCTCGGGTG264810.2462612802331731No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGT262040.24368530596390123No Hit
GGTGCGGAGTGCCCTTCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT258700.24057925756701742RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA256800.23881234380831104RNA PCR Primer, Index 1 (100% over 28bp)
GGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTC249470.2319957765181439No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC227020.21111829552711361No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGC226840.21095090369734143No Hit
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC222590.2069985966054983RNA PCR Primer, Index 1 (100% over 26bp)
GTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCGCTGGAATTCT220910.20543627286095795No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC218600.20328807771221497No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG206030.19159854826645764No Hit
AACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGG205990.19156135008206385No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG204790.1904454045502493No Hit
CTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGGT204170.18986883269214513No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC199940.1859351246924989No Hit
ACAGAGTGGTTCATTTCCACCTTTCGGGCGCTGGAATTCTCGGGTGCCAA198880.1849493728060627No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA198780.18485637734507815No Hit
CGGACGAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCT191880.17843969053714456RNA PCR Primer, Index 27 (100% over 39bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC190870.17750043638120067RNA PCR Primer, Index 1 (100% over 27bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA182770.16996780404145256RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA179210.16665716563040275RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCG179110.16656417016941819RNA PCR Primer, Index 27 (100% over 45bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC178480.16597829876521555RNA PCR Primer, Index 1 (100% over 26bp)
CTGAGAAGACGGTCGAACTTGACTTGGAATTCTCGGGTGCCAAGGAACTC173950.1617656043826157RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA169780.15788769365956018RNA PCR Primer, Index 1 (100% over 28bp)
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCTGGAATTCTC168850.1570228358724039No Hit
CGGTGGGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCT167640.15589759079449092RNA PCR Primer, Index 27 (100% over 39bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCTGGAATTCTCGGGTGCCA167500.15576739714911256No Hit
GATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGC166880.1551908252910084No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC166110.15447476024142737RNA PCR Primer, Index 1 (100% over 33bp)
ACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAG165870.1542515711350645No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGAATTCTCGGGTG165350.15376799473794484No Hit
GGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTG161860.15052245314958423No Hit
AAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGAATTCTCGGGTGCCAAGG161570.15025276631272905Illumina Small RNA Adapter 2 (100% over 21bp)
CTAGAGGAAGTAAAAGTCGTAACAAGGTTTGGAATTCTCGGGTGCCAAGG159570.14839285709303815Illumina Small RNA Adapter 2 (100% over 21bp)
CACCAAGCGTTGGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC154900.1440499690650599RNA PCR Primer, Index 1 (100% over 31bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT153370.14262713851199638RNA PCR Primer, Index 1 (100% over 35bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG152740.14204126710779374No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCTGGAATTCTCGGGTG152680.141985469831203No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT152530.1418459766397262No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAA150610.14006046378882295No Hit
CTAAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAAT150410.13987447286685384No Hit
AAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAATTC144400.13428544566168268No Hit
TAAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAATT141950.13200705686756137No Hit
CGACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGAACTCCA140210.13038893584643027RNA PCR Primer, Index 1 (100% over 28bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA139600.12982166353442456RNA PCR Primer, Index 1 (100% over 34bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTAT138260.12857552435723166RNA PCR Primer, Index 27 (100% over 41bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA136660.12708759698147892No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCC134860.12541367868375713No Hit
GACAGGATTGACAGATTGATAGCTTGGAATTCTCGGGTGCCAAGGAACTC134080.12468831408807768RNA PCR Primer, Index 1 (100% over 26bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG131600.12238202665566097No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGGAATTCTCGGGTGCCAAGG131210.12201934435782125Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT130320.12119168475505879RNA PCR Primer, Index 1 (100% over 30bp)
CTACGGGGATGATTTTACGAACTGAACTTGGAATTCTCGGGTGCCAAGGA127130.11822512954965181RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC126750.11787174679791054RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA126160.11732307357810172No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC125160.11639311896825628No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC123140.11451461065636849No Hit
CACCAAGCGTTGGATTGTTCACCCACTAATATGGAATTCTCGGGTGCCAA122360.11378924606068902No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGTGGAATTCTCGGGTGC118670.11035771355035932No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAA118090.10981833987664896No Hit
GAAAAGAACTTTGAAGAGAGAGTTTGGAATTCTCGGGTGCCAAGGAACTC114900.10685178467124197RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA114100.10610782098336563No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAG113080.10515926728132327No Hit
CACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGG112180.10432230813246238No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTC111090.10330865760773081No Hit
GACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAAC109260.10160684067171365RNA PCR Primer, Index 1 (100% over 24bp)
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTTGGAATTCTCGGGTGCC108180.10060248969308058No Hit
TGAAAAGAACTTTGAAGAGAGAGTTTGGAATTCTCGGGTGCCAAGGAACT108100.10052809332429294RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)