FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002798712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6273814
Sequences flagged as poor quality0
Sequence length76
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC3658785.831827338202886No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG941031.4999328956835507Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA937291.4939716096141837No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT819241.3058085560075579No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGC751441.1977403219158234RNA PCR Primer, Index 26 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG586580.9349655568367186No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG539610.8600988170831969No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG479920.7649573289868014No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA471030.7507873201213807No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG457680.7295083979219021No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA449280.7161194131671739RNA PCR Primer, Index 1 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC408080.6504496307987453No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA399110.6361521077928036RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA368570.5874735846488277No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC355300.5663221765898703No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC348150.5549256002807862No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA328010.522823915404569No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA319750.5096580803957529RNA PCR Primer, Index 1 (100% over 22bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC300510.4789909296003994No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA279750.44590101013514266RNA PCR Primer, Index 1 (100% over 23bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA273640.43616211765283447RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCGCGTATGC269070.42887787237555974RNA PCR Primer, Index 26 (98% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG258880.41263575872666935RNA PCR Primer, Index 15 (100% over 36bp)
CACACGACTCTCGGCAACGGATATCTCGGCTTGGAATTCTCGGGTGCCAA238860.38072534506123384No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT221410.3529113231600427RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC205930.3282373369691865No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA202250.32237168650521036RNA PCR Primer, Index 1 (100% over 32bp)
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG199300.3176696025734904No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC187140.29828745321426486No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC186920.2979367893278315No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT176380.28113680131416074RNA PCR Primer, Index 1 (100% over 35bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA137670.2194358965694552No Hit
AAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAATTC135020.2152119906646898No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC132850.21175316960305168No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA130370.20780023124689384No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC123810.19734407172415377No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAA122170.19473003184346874No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT118710.18921504526592597No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGG118650.18911940966053503RNA PCR Primer, Index 26 (97% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA117180.1867763373284576No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC115970.18484768595307416No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGT115470.18405072257481653No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC111110.17710120191641002No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC110450.17604921025710993RNA PCR Primer, Index 1 (100% over 24bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG109730.17490158299241898Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG109520.17456685837355077Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC98540.15706554258701325No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC96210.1533516932443327No Hit
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT92810.14793234227218083RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTGGAAT90640.14447352121054274No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG90500.1442503714646306No Hit
TTCTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCC89630.14286365518646232No Hit
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT87480.13943671265995453RNA PCR Primer, Index 1 (100% over 25bp)
CTAAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAAT84930.1353721994308406No Hit
CACACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCTGGAATTCTCG83650.13333197318250112No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC82320.1312120505963358No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG81940.13060635842886004No Hit
GGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTC80660.1285661321805205No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA80270.12794450074547953RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC79320.12643027032679005No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCG78980.12588833522957488No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA76840.12247733197063221No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCGCGTATGG76230.12150503664915792RNA PCR Primer, Index 26 (97% over 49bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC72520.11559156838248633RNA PCR Primer, Index 1 (100% over 33bp)
CGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCT68120.10857829065381919No Hit
TCTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCCA67820.10810011262686461No Hit
CACACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGTGGAATTCTC65760.10481662350844319No Hit
AGATAGTACTGATCTGCTGCGACGAGTGACTGTCTGGAATTCTCGGGTGC65680.10468910936792196No Hit
CACACGACTCTCGGCAACGGATATCTCGGCTCTCGCATGGAATTCTCGGG64380.10261700458445214No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTC62830.1001464181118535No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA62800.10009860030915803RNA PCR Primer, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)