FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002798713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10899863
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGAGGTGTCCGAGCGGCTGAGGGAGCATGATTGGAATTCTCGGGTGCC5171404.744463301969942No Hit
CGCCTATCGAGACCTGACGAACTCCGAATGCTTATGGAATTCTCGGGTGC3028982.778915661600517No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC2725852.500811248728539No Hit
CTGGTAGTCCACGCTGTAAACGCTGTCGATTTGGAATTCTCGGGTGCCAA1277771.1722807892172589No Hit
GGGTCTGTAGCTCAGGTGGTTAGAGCGCACCTGGAATTCTCGGGTGCCAA981990.9009195803653679No Hit
AGGGGGTCGTTGGTTCAAGCCCATCCTGGAGCGCCTGGAATTCTCGGGTG945660.8675888862089368No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA760430.6976509704755005No Hit
AGGGGCGTAGTTCAATTGGTAGAGCACCTGGAATTCTCGGGTGCCAAGGA743390.682017746461584RNA PCR Primer, Index 1 (100% over 22bp)
AGGGGGTCGTTGGTTCAAGCCCATCCTGAAGCGCCATGGAATTCTCGGGT625760.5740989588584737No Hit
AGGGGGTCGTTGGTTCAAGCCCATCCTGAAGCGCCTGGAATTCTCGGGTG613020.5624107385569892No Hit
GGTACCTTGGCCGATCGGTTAGGTAACGGTTTGCTGGAATTCTCGGGTGC567180.5203551640970167No Hit
GCCTATCGAGACCTGACGAACTCCGAATGCTTATGGAATTCTCGGGTGCC431020.395436162821496No Hit
GTTAAGTGACTAAGCGTACAAGGTGGATGCCTGGAATTCTCGGGTGCCAA415180.38090387007616516No Hit
CTATCGAGACCTGACGAACTCCGAATGCTTATGGAATTCTCGGGTGCCAA413600.3794543105725274No Hit
AGGGGGTCGTTGGTTCAAGCCCATCCTGGAGCGCCATGGAATTCTCGGGT411770.37777539038793423No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA379120.3478208854551658No Hit
CGCGGGTTGGAGCAGTTGGTAGCTCGCCTGGAATTCTCGGGTGCCAAGGA373760.3429033924554832RNA PCR Primer, Index 1 (100% over 22bp)
GGAGAGATGGTAGAGTGGTCGATTACAACGGTCTTTGGAATTCTCGGGTG369010.3385455395173315No Hit
GGAAGGATGGGTGAGTGGCTGAAACCAGCAGTTTGCTGGAATTCTCGGGT367240.3369216658961677No Hit
GGAGAGATGGTAGAGTGGTCGATTACAACGGTCTTGGAATTCTCGGGTGC358930.32929771686121195No Hit
AGAAAGGGCGCATGGCGGATGCCTTGGCTTGGAATTCTCGGGTGCCAAGG341010.3128571432503326Illumina Small RNA Adapter 2 (100% over 21bp)
GGAAGGATGGGTGAGTGGCTGAAACCAGCAGTTTTGGAATTCTCGGGTGC339270.3112607929108834No Hit
CGGGGTCGTTGGTTCGAATCCGACAAGAGGCTTGGAATTCTCGGGTGCCA328650.30151755118390017No Hit
GGCTTCGTAGCTCAACTGAATAGAGCATCTGACTTGGAATTCTCGGGTGC324730.2979211757065203No Hit
GGAGAGGTGTCCGAGCGGCTGATGGAGCATGATTGGAATTCTCGGGTGCC319740.2933431365146516No Hit
ACGGGTTTAGCTCAGTTGGTAGAGCACTGGTCTTGGAATTCTCGGGTGCC310660.28501275658235337No Hit
GCGGAAATAGCTCAGTTGGTAGAGCACAACCTTGGAATTCTCGGGTGCCA305840.28059068265353426No Hit
GATCGGTCGTAGGTTCGAGTCCTACTCGAGGAGCCATGGAATTCTCGGGT276610.25377383183623503No Hit
GGAGAGGTGTCCGAGCGGCTGAAGGAGCATGATTGGAATTCTCGGGTGCC274410.25175545784382797No Hit
GCGGCAATAGCTCAGTTGGTAGAGCACGTGGAATTCTCGGGTGCCAAGGA272990.25045268917600155RNA PCR Primer, Index 1 (100% over 22bp)
GGCTCCGTAGCTCAACTGAATAGAGCATCTGACTTGGAATTCTCGGGTGC267960.24583795227518No Hit
TTAAGTGACGATAGCCTAGGAGATACACCTTGGAATTCTCGGGTGCCAAG260870.2393332833632863No Hit
GTTAAGTGACTAAGCGTACAAGGTGGATGCCTTGGAATTCTCGGGTGCCA226320.20763563725525722No Hit
GGTGCCTGGGCCGGTCGGTTAGGTAACGGTTTGCTGGAATTCTCGGGTGC220860.20262639998319246No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA201210.1845986504601021No Hit
GGAAGGATGGGTGAGTGGCTGAAACCTGGAATTCTCGGGTGCCAAGGAAC195550.17940592464327304RNA PCR Primer, Index 1 (100% over 24bp)
GGTGGACGTAGTTCAGTTGGTTAGAGCGTCTGGAATTCTCGGGTGCCAAG189900.17422237325368217No Hit
GCGGAATTAGCACATCGGTAGTGCACGTGGAATTCTCGGGTGCCAAGGAA187830.1723232668153719RNA PCR Primer, Index 1 (100% over 23bp)
GGAGGGGTTCCCGAGCGGCCAAAGGGGGCAGACTGTTGGAATTCTCGGGT187540.1720572084254637No Hit
TTCAAAGAGTGCGTAACAGCTCACTAGTCGAGGGTCCGGGCATGGAATTC185600.1702773695412502No Hit
CGGGAAGTAGCTCAGCTTGGTAGAGTATGGAATTCTCGGGTGCCAAGGAA185260.16996543901515093RNA PCR Primer, Index 1 (100% over 23bp)
TCTAAAGAGCAGTTCGACTCTGCTAGGGACTACCTGGAATTCTCGGGTGC183570.16841496081189275No Hit
CGGCGGTAACACGGGTTCGAATCCCGTACGGACTTGGAATTCTCGGGTGC181250.16628649369262713No Hit
GGTGGTCAGGTAGAGAATACTCAGGCGCTTGGAATTCTCGGGTGCCAAGG174910.16046990682359952Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA173960.1595983362359692RNA PCR Primer, Index 1 (100% over 23bp)
CGGGAAGTAGCTCAGCTTGGTAGAGTACTTGGAATTCTCGGGTGCCAAGG169740.15572672794144293Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGTCGTTGGTTCGAATCCGACAAGAGGCTCTGGAATTCTCGGGTGCC163110.1496440826825071No Hit
GGTGCGTTAGTTCAGTTGGTTAGAATGCCTGGAATTCTCGGGTGCCAAGG159350.14619449804093868Illumina Small RNA Adapter 2 (100% over 21bp)
GGAAGGATGGGTGAGTGGCTGAAACCATGGAATTCTCGGGTGCCAAGGAA154290.14155223785840243RNA PCR Primer, Index 1 (100% over 23bp)
AGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTTGGAATTCTCGGGTGCC153850.14114856305992102No Hit
GGTGACTGTAGTTCAGCTGGTTAGAGCGTCTGGAATTCTCGGGTGCCAAG152240.1396714802745686No Hit
GGAGAGGTGTCCGAGCGGCTGAGGAGCATGATTGGAATTCTCGGGTGCCA147810.13560720900803983No Hit
AGAGGGTCGCGTGTTCAAGTCATGTAGCCGGCACCATGGAATTCTCGGGT147430.1352585807729877No Hit
GCGGAAATAGCTCAGTTGATAGAGCACTTGGAATTCTCGGGTGCCAAGGA146400.13431361476745168RNA PCR Primer, Index 1 (100% over 22bp)
TGATGGGGTGACTGCGTACCTTTTGTATAATGGGTCTGGAATTCTCGGGT144200.13229524077504462No Hit
ATGTGGAGGTCGGCAGTTCAAGTCTGCCTGGGACTTGGAATTCTCGGGTG142840.1310475186706475No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA140870.12924015650471937RNA PCR Primer, Index 1 (100% over 23bp)
GGAAGGTCGTCATCTCCGGTGAGGTGGCAGGACTTGGAATTCTCGGGTGC140680.12906584238719332No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA140180.12860712102528263No Hit
GGTTCCGTAGCTCAACTGAATAGAGCATCTGACTTGGAATTCTCGGGTGC137770.12639608406087305No Hit
TCGGGGTCGTTGGTTCGAATCCGACAAGAGGCTCCTGGAATTCTCGGGTG137380.1260382813985827No Hit
GATCGGTCGTAGGTTCGAGTCCTACTCGGGGAGCCATGGAATTCTCGGGT134850.1237171513073146No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA134000.12293732499206642No Hit
GCGGAAATAGCTCAGTTGGTAGAGCACAACTGGAATTCTCGGGTGCCAAG133280.122276766230915No Hit
GGTGGATGTAGTTCAGTTGGTTAGAGCGTCTGGAATTCTCGGGTGCCAAG131870.12098317199032685No Hit
TCGGGGTCGTTGGTTCGAATCCGACAAGAGGCTCTGGAATTCTCGGGTGC131230.12039600864708117No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG128480.11787304115657234Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGTAACACGGGTTCGAATCCCGTACGGACTGGAATTCTCGGGTGCCAA125150.1148179568862471No Hit
GGAAGGATGGGTGAGTGGCTGAAACCAGCATGGAATTCTCGGGTGCCAAG124390.11412070041614283No Hit
CCTATCGAGACCTGACGAACTCCGAATGCTTATGGAATTCTCGGGTGCCA124090.11384546759899641No Hit
AATACTAATAGCTCGAGGACTTATCCTGGAATTCTCGGGTGCCAAGGAAC119970.11006560357685229RNA PCR Primer, Index 1 (100% over 24bp)
GGTACCTTGGCCGATCGGTTAGGTAACTGGAATTCTCGGGTGCCAAGGAA119540.1096711032056091RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGAGTGGAGCAGTTGGTAGCTCGCCAGGCTGGAATTCTCGGGTGCCA119510.10964357992389445No Hit
CGGGATGTAGTTCAGTAGGTTTAGAATGCTGGTCTTGGAATTCTCGGGTG118680.1088821024631227No Hit
GGAGAGATGGTAGAGTGGTCGATTACAACGTGGAATTCTCGGGTGCCAAG118180.10842338110121201No Hit
AAGAAAGGGCGCATGGCGGATGCCTTGGCTTGGAATTCTCGGGTGCCAAG114720.10524902927678999No Hit
GGGGCCTTAGCTCAGCTGGGAGAGCGCCTGGAATTCTCGGGTGCCAAGGA114400.10495544760516715RNA PCR Primer, Index 1 (100% over 22bp)
GGGGATATAGCTCAGCTGGGAGAGCGCCTGGAATTCTCGGGTGCCAAGGA112300.10302881788514223RNA PCR Primer, Index 1 (100% over 22bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA111810.10257927095046973RNA PCR Primer, Index 1 (100% over 22bp)
GAATTGAAGAGTTTGATCATGGCTTGGAATTCTCGGGTGCCAAGGAACTC109860.10079025763901804RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)