FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8832132
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC3262803.6942382654607067No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG2641962.9913049306781194Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA2630182.9779672676993507No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG1972252.2330395424343745No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1294441.465603095605908No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1179591.335566542710186No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA1098811.2441050473430424RNA PCR Primer, Index 1 (100% over 23bp)
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGT844100.9557148828844496No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA840470.9516048899631482No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG830920.9407920986688152No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA788840.8931478832064558RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA775440.8779760085107424No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA706490.799908787595113No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA662620.7502378814084754RNA PCR Primer, Index 1 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA636710.7209018162319132No Hit
CGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC626380.709205885962755RNA PCR Primer, Index 1 (100% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA611610.6924828569138233No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC605940.6860631159045177No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA599410.6786696575639948RNA PCR Primer, Index 1 (100% over 23bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA545700.617857613541102RNA PCR Primer, Index 1 (100% over 28bp)
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCG543580.6154572870967056No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC529680.5997192976735402No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA494560.5599553992173124No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG473680.5363144481989173No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC463460.5247430631697987RNA PCR Primer, Index 1 (100% over 31bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG460880.5218219111761463No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA445420.5043176438033309No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA399120.4518954200412766No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTG395980.44834021955287806No Hit
AAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGAATTCTCGGGTGCCAAGG379790.43000942467798264Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC376540.4263296789495447No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA372870.4221743968500471No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA368040.4167057285828608No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA362300.410206731511712RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA345280.3909361861892463RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA338770.38356537243782135No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT332080.37599075738451376No Hit
GAACGCGCCCGATCTCGTCTGATCTCGGAAGTGGAATTCTCGGGTGCCAA318340.36043392467413304No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC318160.3602301233722503RNA PCR Primer, Index 1 (100% over 26bp)
CGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG299600.33921594468923244Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCTCGAAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA290700.32913910254058704RNA PCR Primer, Index 1 (100% over 37bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC282830.3202284567304927RNA PCR Primer, Index 1 (100% over 24bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA280780.3179073863479395RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC270010.3057132751186237No Hit
TTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGAATTCTCGGGTGCCAA267880.3033016263796782No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC266070.30125229106630197No Hit
CGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG254480.2881297516839649No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG251880.2851859551012145Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG249860.2828988516023085No Hit
GGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTC244300.27660365583304236No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC239460.2711236652713071No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG238110.2695951555071867Illumina Small RNA Adapter 2 (100% over 21bp)
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGT233840.2647605357347467No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA233680.26457937902196205No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA222990.2524758461490385RNA PCR Primer, Index 1 (100% over 22bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA215410.24389354688086634RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAATTCTCGGGTGCCAA212250.24031570180336978No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC211060.23896834875203407No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC205740.23294488805194485No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC201410.22804233451221065No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA183500.20776410497488035No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT174540.1976193290589407No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC163910.1855837299533114RNA PCR Primer, Index 1 (100% over 27bp)
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGC163810.18547050700782097No Hit
AACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGG161150.1824587766577764No Hit
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT156840.17757886770714026RNA PCR Primer, Index 1 (100% over 30bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG156790.17752225623439505Illumina Small RNA Adapter 2 (100% over 21bp)
CTCTCTCTCCCCCGCTCCCCGTCCTTGGAATTCTCGGGTGCCAAGGAACT154090.1744652367061543RNA PCR Primer, Index 1 (100% over 25bp)
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG151330.1713402834106193No Hit
CGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG151040.17101193686869715RNA PCR Primer, Index 1 (100% over 45bp)
GGGTCGGGCCTGGTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGG147630.1671510344274746No Hit
AAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAATTC144040.1630863306843693No Hit
CTGAGAAGACGGTCGAACTTGACTATCTAGTGGAATTCTCGGGTGCCAAG141820.16057278129448246No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG139700.15817245485008602No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGGAATTCTCGGGTGCCAAGG138100.15636088772223966Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT136410.15444741994345193No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG136180.154187007168824Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT132150.14962412246556098RNA PCR Primer, Index 1 (100% over 43bp)
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGTGGAATTCTCGGGT132150.14962412246556098No Hit
GTTGAACATGGGTCAGTCGGTCCTGAGAGATGGAATTCTCGGGTGCCAAG130710.14799371205049924No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG129940.1471218953702232No Hit
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAG128230.14518578300233737RNA PCR Primer, Index 1 (100% over 29bp)
CGCGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA128200.14515181611869024No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAA125270.14183438381582159No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA122230.1383924062729135No Hit
TGGTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAA121630.13771306859997112RNA PCR Primer, Index 1 (100% over 23bp)
AAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGGAGACCGCCTGGAATTC121430.1374866227089903No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC117110.1325953914638051RNA PCR Primer, Index 1 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA116520.13192737608541177RNA PCR Primer, Index 1 (100% over 22bp)
GACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAAC115170.13039886632129138RNA PCR Primer, Index 1 (100% over 24bp)
GCGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA114970.13017242043031058No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAA113160.1281230851169344No Hit
CGCGTGGGTTCGAATCCCATCCTCGTCGCCTGGAATTCTCGGGTGCCAAG108200.12250722702061065No Hit
CGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGAATTCTCG108020.12230342571872793No Hit
CTGGACGCGAGCCGGGCCCTTCCCGTGGATCTGGAATTCTCGGGTGCCAA107250.12143160903845186No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC107230.1214089644493538No Hit
AGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGG106300.12035599105629309No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA104160.11793302002279858RNA PCR Primer, Index 1 (100% over 28bp)
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCG102900.11650641090961955No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC100110.11334749073043746RNA PCR Primer, Index 1 (100% over 26bp)
CTAAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAAT99410.11255493011200467No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC99020.11211336062459211RNA PCR Primer, Index 1 (100% over 26bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA97500.11039237185313806RNA PCR Primer, Index 1 (100% over 32bp)
ACAGAGTGGTTCATTTCCACCTTTCGGGCGCTGGAATTCTCGGGTGCCAA97010.10983757942023512No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAG96710.10949791058376393No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC96230.10895444044541RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC95790.10845625948525225No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA94100.10654279170646452No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTC94030.10646353564462126No Hit
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA93810.10621444516454238RNA PCR Primer, Index 1 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA93670.10605593304085581No Hit
CTCTCTCTCTCCCCCGCTCCCCGTCCTTGGAATTCTCGGGTGCCAAGGAA90550.1025233771415554RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG89880.10176478340676973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)