FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002798718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5666268
Sequences flagged as poor quality0
Sequence length76
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA4431537.820897281949954No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1395672.4631203465843834RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA948351.673676571598802No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC722721.275477968920637RNA PCR Primer, Index 8 (100% over 50bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC476170.8403591217358586RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG457640.8076568210328209RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG294510.5197600960632289Illumina Small RNA Adapter 2 (100% over 21bp)
GATTGGTCAGGCTTGGTTGTTAGTGTCGGAAAGGCGCCTGGAATTCTCGG283180.49976457167221877No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA225430.39784563666949746RNA PCR Primer, Index 1 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT215940.38109739955822775RNA PCR Primer, Index 8 (100% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC210350.371231999615973No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT204820.3614724894763185RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG203830.35972530773341466No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGCGTATGC183990.32471107967360524RNA PCR Primer, Index 8 (98% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA178190.3144750654222497No Hit
GGATTGGTCAGGCTTGGTTGTTAGTGTCGGAAAGGCGCCTGGAATTCTCG145690.2571180890137918No Hit
GCCTTGGCTGGAAGAATTTGGACCTGGCTGGAATTCTCGGGTGCCAAGGA121160.2138268080507311RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC115600.20401435300977647RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC114080.20133181134390393RNA PCR Primer, Index 5 (100% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGG102420.1807538930385926RNA PCR Primer, Index 8 (97% over 49bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT89710.15832290318777723RNA PCR Primer, Index 1 (100% over 25bp)
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT85030.15006349858495927RNA PCR Primer, Index 1 (100% over 25bp)
CACATTATTGTCTGGGTCAAGTCCCTTGGAACAGGGCTGGAATTCTCGGG84470.14907519376069048No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA84230.14865163455028954RNA PCR Primer, Index 1 (100% over 22bp)
AAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAATTC79080.13956275982710312No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC71550.12627358960077426No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA65530.11564931273988452RNA PCR Primer, Index 1 (100% over 28bp)
GAGCTAAGATCCGGAGATGTCCGAATGAGGGAACTCTTGGAATTCTCGGG64570.11395507589828086No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA62510.11031952600900627RNA PCR Primer, Index 1 (100% over 23bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT60950.1075663911414003RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC59860.10564272639416279No Hit
GAGTTAAATCCTCTGTAGACGACTTAAATGGAATTCTCGGGTGCCAAGGA58950.10403673105472597RNA PCR Primer, Index 1 (100% over 22bp)
GATTGGTCAGGCTTGGTTGTTAGTGTCGGAAAGGCGCCTTGGAATTCTCG57110.10078944377498557No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)