FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9609895
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCG8432568.774872150007882RNA PCR Primer, Index 29 (100% over 45bp)
GAGAGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT5982756.225614327731989RNA PCR Primer, Index 29 (100% over 41bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA4749404.94219759945348No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA4729284.921260846242337No Hit
GAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC2351082.446519967179662RNA PCR Primer, Index 29 (100% over 44bp)
AGAGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC1953012.032290675392395RNA PCR Primer, Index 29 (100% over 42bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1903101.9803546240619696No Hit
AGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC1720681.790529449073065RNA PCR Primer, Index 29 (100% over 44bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCG1700781.7698216265630375RNA PCR Primer, Index 29 (100% over 45bp)
CTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA1477331.537300875816021RNA PCR Primer, Index 29 (100% over 40bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCG1426251.4841473293932972RNA PCR Primer, Index 29 (100% over 45bp)
AGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCT1332501.3865916328950523RNA PCR Primer, Index 29 (100% over 43bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1289401.3417420273582594No Hit
GAGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCT1116551.1618753378678954RNA PCR Primer, Index 29 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGC980391.0201880457590846RNA PCR Primer, Index 29 (100% over 50bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA907020.9438396569369385RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA672740.7000492721304448RNA PCR Primer, Index 1 (100% over 28bp)
GAAAGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA640760.6667710729409635RNA PCR Primer, Index 29 (100% over 40bp)
GAGGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT608760.6334720618695625RNA PCR Primer, Index 29 (100% over 41bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA582580.6062293084367727No Hit
GAGAGAGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA565460.5884143375135733RNA PCR Primer, Index 29 (100% over 39bp)
AGGGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC549680.5719937626789887RNA PCR Primer, Index 29 (100% over 42bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGT534810.556520128471747RNA PCR Primer, Index 29 (100% over 46bp)
AAATGGATTTTTGGAGCAGGGAGATTGGAATTCTCGGGTGCCAAGGAACT519100.5401723952238812RNA PCR Primer, Index 1 (100% over 25bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA476060.495385225332847RNA PCR Primer, Index 29 (100% over 39bp)
CCTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA455950.47445887806266357RNA PCR Primer, Index 29 (100% over 39bp)
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC432030.4495678672867914RNA PCR Primer, Index 29 (100% over 44bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC418290.4352701044080086No Hit
GGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC417680.43463534200946RNA PCR Primer, Index 29 (100% over 44bp)
TGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT388760.40454136075368147RNA PCR Primer, Index 29 (100% over 41bp)
AGAGAGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA380620.39607092481239387RNA PCR Primer, Index 29 (100% over 40bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATGTCG371250.38632055813304933RNA PCR Primer, Index 29 (97% over 45bp)
GAGGGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT369480.38447870658316247RNA PCR Primer, Index 29 (100% over 41bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC361630.37631004292970943RNA PCR Primer, Index 1 (100% over 26bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAGCTCG345530.3595564779844108RNA PCR Primer, Index 29 (97% over 45bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA345390.3594107948109735No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA333310.3468404181315196RNA PCR Primer, Index 1 (100% over 28bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATG330660.34408284377716924RNA PCR Primer, Index 29 (100% over 49bp)
CCCTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT325590.33880703171054416RNA PCR Primer, Index 29 (100% over 38bp)
AAAGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT320510.33352081370295933RNA PCR Primer, Index 29 (100% over 41bp)
GGGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC307670.3201595855105597RNA PCR Primer, Index 29 (100% over 42bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA293900.3058306048088975RNA PCR Primer, Index 1 (100% over 28bp)
GGAGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT292800.3046859513033181RNA PCR Primer, Index 29 (100% over 41bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC292370.30423849584204615RNA PCR Primer, Index 1 (100% over 27bp)
AGAGGGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA267760.2786294751399469RNA PCR Primer, Index 29 (100% over 40bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA265600.2763817918926273RNA PCR Primer, Index 1 (100% over 28bp)
GAGAAGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA255760.26614234598817155RNA PCR Primer, Index 29 (100% over 40bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATATCG253000.26327030628326326RNA PCR Primer, Index 29 (97% over 45bp)
AGAGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATG249170.25928483089565496RNA PCR Primer, Index 29 (97% over 41bp)
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA224780.2339047408946716RNA PCR Primer, Index 1 (100% over 28bp)
AGGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC222770.23181314676174922RNA PCR Primer, Index 29 (100% over 42bp)
GAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATGTC221230.23021063185393806RNA PCR Primer, Index 29 (97% over 44bp)
AGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC220190.2291284139941175RNA PCR Primer, Index 29 (100% over 44bp)
TACTGCGGGCTGTCAGATGCTCACCTGGAATTCTCGGGTGCCAAGGAACT212520.22114705727794112RNA PCR Primer, Index 1 (100% over 25bp)
GGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCT202420.2106370569085302RNA PCR Primer, Index 29 (100% over 43bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC200590.20873276971288446RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA200010.20812922513721532No Hit
AGGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCT195720.20366507646545567RNA PCR Primer, Index 29 (100% over 43bp)
GAGGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC183690.1911467294908009RNA PCR Primer, Index 29 (100% over 42bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA177430.1846326104499581RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA173780.18083444199962642RNA PCR Primer, Index 29 (100% over 40bp)
GAGAGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAG171240.17819133299583398RNA PCR Primer, Index 29 (97% over 40bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT156030.16236389679595875RNA PCR Primer, Index 1 (100% over 30bp)
AGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATGTC155360.1616666987516513RNA PCR Primer, Index 29 (97% over 44bp)
TGCTGCGGGCTGTCAGATGCTCACCTGGAATTCTCGGGTGCCAAGGAACT154970.1612608670542186RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC141710.14746258934150686RNA PCR Primer, Index 1 (100% over 26bp)
AGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATGT132360.13773303454408192RNA PCR Primer, Index 29 (97% over 43bp)
AGAGTTCTACAGTCCGACGATCGGGGGCTGGAATTCTCGGGTGCCAAGGA131680.13702543055881464Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCT131520.13685893550345762RNA PCR Primer, Index 29 (100% over 43bp)
GAAGAGGGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT129370.1346216581970979RNA PCR Primer, Index 29 (100% over 38bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA126250.13137500461763632RNA PCR Primer, Index 1 (100% over 28bp)
GGAGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC122590.12756643022634484RNA PCR Primer, Index 29 (100% over 42bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC121020.12593269749565422RNA PCR Primer, Index 29 (100% over 44bp)
GAGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATGT118130.12292538055826832RNA PCR Primer, Index 29 (97% over 43bp)
AGAGGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT114800.11946020221865067RNA PCR Primer, Index 29 (100% over 41bp)
AGAGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATA107430.11179102373126866RNA PCR Primer, Index 29 (97% over 41bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG102380.1065360235465632No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG99830.10388250860181095No Hit
AAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC99170.10319571649846329RNA PCR Primer, Index 29 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph